Overview
- External ontology
- mediadive.medium:286 (source link unavailable) — CLOSTRIDIUM AMINOBUTYRICUM MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium286.pdf
Composition
flowchart LR
CultureMech_001383["clostridium_aminobutyricum_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_001383 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_K2HPO4["K2HPO4"]
CultureMech_001383 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001383 --> ing2_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
ing3_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_001383 --> ing3_FeCl3_x_6_H2O
CHEBI_86254["CHEBI:86254"]
ing3_FeCl3_x_6_H2O -.-> CHEBI_86254
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001383 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001383 --> ing5_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing5_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing6_Yeast_extract["Yeast extract"]
CultureMech_001383 --> ing6_Yeast_extract
ing7_gamma_Aminobutyric_acid["gamma-Aminobutyric acid"]
CultureMech_001383 --> ing7_gamma_Aminobutyric_acid
CHEBI_16865["CHEBI:16865"]
ing7_gamma_Aminobutyric_acid -.-> CHEBI_16865
ing8_Agar["Agar"]
CultureMech_001383 --> ing8_Agar
CHEBI_2509["CHEBI:2509"]
ing8_Agar -.-> CHEBI_2509
ing9_Sodium_resazurin["Sodium resazurin"]
CultureMech_001383 --> ing9_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing9_Sodium_resazurin -.-> CHEBI_8806
ing10_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_001383 --> ing10_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing10_Na2S_x_9_H2O -.-> CHEBI_76209
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.3 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
7 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.2 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 4 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
0.01 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.01 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 6 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.001 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 7 | Yeast extract | 3 G_PER_L | ||
| 8 |
gamma-Aminobutyric acid
C4H9NO2 103.121 g/mol
|
5 G_PER_L |
CHEBI:16865
gamma-aminobutyric acid
|
|
| 9 | Agar | 1.5 G_PER_L |
CHEBI:2509
agar
|
|
| 10 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.0005 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 11 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.3 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Preparation
- Dissolve ingredients (except sulfide), adjust pH to 7.2, bring medium to the boil, then cool to room temperature under 100% N2 gas. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add sulfide from a sterile anoxic stock solution prepared under 100% N2 gas atmosphere.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.665916Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 286 |
| 2026-03-13T06:19:03.589988Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001383 |
| 2026-03-17T06:30:57.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:15.533029+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CLOSTRIDIUM AMINOBUTYRICUM MEDIUM" to "clostridium_aminobutyricum_medium" for consistent matching |
| 2026-06-05T07:40:53.754292+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:54.635163+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |