Overview
- External ontology
- mediadive.medium:289 (source link unavailable) — RUMINICLOSTRIDIUM PAPYROSOLVENS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium289.pdf
Composition
flowchart LR
CultureMech_001386["ruminiclostridium_papyrosolvens_medium"]
ing0_Sea_water["Sea water"]
CultureMech_001386 --> ing0_Sea_water
ing1_NH4Cl["NH4Cl"]
CultureMech_001386 --> ing1_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing1_NH4Cl -.-> CHEBI_31206
ing2_Yeast_extract["Yeast extract"]
CultureMech_001386 --> ing2_Yeast_extract
ing3_K2HPO4["K2HPO4"]
CultureMech_001386 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_Sodium_resazurin["Sodium resazurin"]
CultureMech_001386 --> ing4_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing4_Sodium_resazurin -.-> CHEBI_8806
ing5_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_001386 --> ing5_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing5_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing6_Cellobiose["Cellobiose"]
CultureMech_001386 --> ing6_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing6_Cellobiose -.-> CHEBI_17057
ing7_Cellulose["Cellulose"]
CultureMech_001386 --> ing7_Cellulose
CHEBI_18246["CHEBI:18246"]
ing7_Cellulose -.-> CHEBI_18246
ing8__NH4_2SO4["(NH4)2SO4"]
CultureMech_001386 --> ing8__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing8__NH4_2SO4 -.-> CHEBI_62946
ing9_NaCl["NaCl"]
CultureMech_001386 --> ing9_NaCl
CHEBI_26710["CHEBI:26710"]
ing9_NaCl -.-> CHEBI_26710
ing10_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001386 --> ing10_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing10_MgSO4_x_7_H2O -.-> CHEBI_31795
ing11_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001386 --> ing11_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing11_CaCl2_x_2_H2O -.-> CHEBI_86158
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Sea water | 200 G_PER_L | ||
| 2 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 3 | Yeast extract | 0.6 G_PER_L | ||
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1.65 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 5 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.0005 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 6 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 7 |
Cellobiose
C12H22O11 342.297 g/mol
|
5 G_PER_L |
CHEBI:17057
cellobiose
|
|
| 8 |
Cellulose
(C6H10O5)n.H2O 180.156 g/mol
|
5 G_PER_L |
CHEBI:18246
(1->4)-beta-D-glucan
|
Optional ingredient (Lens tissue or MN 301) |
| 9 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
6 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 10 |
NaCl
Cl.Na 58.443 g/mol
|
6 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 11 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
1.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 12 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.8 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Preparation
- Dissolve ingredients (except cysteine and cellobiose) and sparge medium with 100% N2 gas for 30 - 45 m in to make it anoxic. Add cysteine, adjust pH to 7.2 and dispense under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add cellobiose from an anoxic stock solution sterilized by filtration.
- Note: Some strains can be adapted to cellulose as substrate using 5.0 g/l cellulose (Lens tissue, OXOID; or cellulose powder MN 301, MACHEREY-NAGEL). If necessary adjust pH of completed medium to 7.2.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.677668Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 289 |
| 2026-03-13T06:19:03.630313Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001386 |
| 2026-03-17T06:30:50.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:15.585334+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "RUMINICLOSTRIDIUM PAPYROSOLVENS MEDIUM" to "ruminiclostridium_papyrosolvens_medium" for consistent matching |
| 2026-06-05T07:41:44.831552+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:11.143197+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |