medium_for_chlorobium_ferrooxidans

Overview

External ontology

mediadive.medium:29a (source link unavailable) — MEDIUM FOR CHLOROBIUM FERROOXIDANS

Applications

• Microbial cultivation

pH

6.8

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium29a.pdf

Composition

flowchart LR
    CultureMech_001398["medium_for_chlorobium_ferrooxidans"]
    ing0_Bicarbonate["Bicarbonate"]
    CultureMech_001398 --> ing0_Bicarbonate
    ing1_FeSO4["FeSO4"]
    CultureMech_001398 --> ing1_FeSO4
    CHEBI_75832["CHEBI:75832"]
    ing1_FeSO4 -.-> CHEBI_75832
    ing2_Acetate["Acetate"]
    CultureMech_001398 --> ing2_Acetate
    CHEBI_30089["CHEBI:30089"]
    ing2_Acetate -.-> CHEBI_30089
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001398 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_Yeast_extract["Yeast extract"]
    CultureMech_001398 --> ing4_Yeast_extract
    ing5_NaHCO3["NaHCO3"]
    CultureMech_001398 --> ing5_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing5_NaHCO3 -.-> CHEBI_32139
    ing6_Resazurin["Resazurin"]
    CultureMech_001398 --> ing6_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing6_Resazurin -.-> CHEBI_8806
    ing7_Ammonium_chloride["Ammonium chloride"]
    CultureMech_001398 --> ing7_Ammonium_chloride
    CHEBI_31206["CHEBI:31206"]
    ing7_Ammonium_chloride -.-> CHEBI_31206
    ing8_Ammonium_acetate["Ammonium acetate"]
    CultureMech_001398 --> ing8_Ammonium_acetate
    CHEBI_62947["CHEBI:62947"]
    ing8_Ammonium_acetate -.-> CHEBI_62947
    ing9_Pyruvic_acid_sodium_salt["Pyruvic acid sodium salt"]
    CultureMech_001398 --> ing9_Pyruvic_acid_sodium_salt
    CHEBI_50144["CHEBI:50144"]
    ing9_Pyruvic_acid_sodium_salt -.-> CHEBI_50144
    ing10_Dextrose["Dextrose"]
    CultureMech_001398 --> ing10_Dextrose
    CHEBI_17634["CHEBI:17634"]
    ing10_Dextrose -.-> CHEBI_17634
    ing11_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001398 --> ing11_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing11_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing12_KCl["KCl"]
    CultureMech_001398 --> ing12_KCl
    CHEBI_32588["CHEBI:32588"]
    ing12_KCl -.-> CHEBI_32588
    ing13_KH2PO4["KH2PO4"]
    CultureMech_001398 --> ing13_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing13_KH2PO4 -.-> CHEBI_63036
    ing14_Vitamin_B12["Vitamin B12"]
    CultureMech_001398 --> ing14_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing14_Vitamin_B12 -.-> CHEBI_176843
    ing15_HCl["HCl"]
    CultureMech_001398 --> ing15_HCl
    CHEBI_17883["CHEBI:17883"]
    ing15_HCl -.-> CHEBI_17883
    ing16_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001398 --> ing16_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing16_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing17_ZnCl2["ZnCl2"]
    CultureMech_001398 --> ing17_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing17_ZnCl2 -.-> CHEBI_49976
    ing18_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001398 --> ing18_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing18_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing19_H3BO3["H3BO3"]
    CultureMech_001398 --> ing19_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing19_H3BO3 -.-> CHEBI_33118
    ing20_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001398 --> ing20_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing20_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing21_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001398 --> ing21_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing21_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing22_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001398 --> ing22_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing22_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing23_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001398 --> ing23_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing23_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (24)

Preparation

1. Mix solution D, C and E. Bubble with CO2 in an ice bath under sterile conditions.
2. Fill 50 ml in each bottle of solution A. Before using add 4 ml solution B and 0.1 ml solution F. To final medium add 10 mM bicarbonate, 10 mM FeSO4 and 2mM acetate.
3. Adjust pH to 6.8.
4. Fill in sterile, N2 gassed screw-cap tubes under N2 gas.
5. At 25C in light the incubation time is about 4 weeks.
6. Fill 10x 46ml in 100 ml screw-cap bottles. Bubble with N2/CO2 and autoclave 121°C 15 min.
7. Prepare in a screw-cap bottle, bubble with N2 to replace air, close tightly and autoclave.
8. Bubble with CO2 and filter sterilize into sterile, gas-tight, 100 ml screw-cap bottle.
9. Autoclave in a cotton-stoppered Erlenmeyer flask with an outlet tube for medium, connected to a glass outlet at the bottom of the vessel and has, at the other end, a silicon rubber tube with a pinch cock and a bell for aseptic dispensing of the medium into bottles.
10. Cool to room temperature under an atmosphere of N2/CO2 in an ice bath.
11. Filter sterilize into sterile, gas-tight, 100 ml screw-cap bottle.
12. Filter sterilized

Target organisms

No target organisms are recorded.

Provenance