Overview
- External ontology
- mediadive.medium:2 (source link unavailable) — BACILLUS PASTEURII MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium2.pdf
Composition
flowchart LR
CultureMech_001399["bacillus_pasteurii_medium"]
ing0_Urea["Urea"]
CultureMech_001399 --> ing0_Urea
CHEBI_16199["CHEBI:16199"]
ing0_Urea -.-> CHEBI_16199
Ingredients (1)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Urea
CH4N2O 60.056 g/mol
|
20 G_PER_L |
CHEBI:16199
urea
|
Preparation
- To medium 1 add 20 g/l urea before autoclaving. Do not adjust pH; pH raises to about 8 due to heat degraded urea.
- For sporulation enhancement add 10 mg/l MnSO4 x H2O.
Solutions
Main sol. 1
Dose: 1 L
| Ingredient | Concentration | Notes |
|---|---|---|
| Peptone | 5 G_PER_L | |
| Meat extract | 3 G_PER_L | |
| Agar | 15 G_PER_L | (for solid medium) |
| Distilled water | 1 L |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:09.960353Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 2 |
| 2026-03-13T06:19:03.783028Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001399 |
| 2026-03-17T06:30:06.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:15.752163+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "BACILLUS PASTEURII MEDIUM" to "bacillus_pasteurii_medium" for consistent matching |
| 2026-06-05T07:40:50.308108+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-10-07T11:13:51.120398+00:00 | codex | RESTORED_DSMZ2_BASE_MEDIUM_AND_PREPARATION_STAGE | Exact source identity, base recipe, original PDFs, and preparation-stage instructions verified. Three ingredients relocated with their identifiers and amounts preserved; two explicit batch-volume slots added. Conditional sporulation supplement not promoted to a mandatory ingredient. |