nutrient_broth_with_10_horse_serum

CultureMech:001402 bacterial COMPLEX SOLID_AGAR
Original name: NUTRIENT BROTH WITH 10% HORSE SERUM

Overview

External ontology
mediadive.medium:302 (source link unavailable) — NUTRIENT BROTH WITH 10% HORSE SERUM
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium302.pdf

Composition

flowchart LR
    CultureMech_001402["nutrient_broth_with_10_horse_serum"]
    ing0_Peptone["Peptone"]
    CultureMech_001402 --> ing0_Peptone
    ing1_Meat_extract["Meat extract"]
    CultureMech_001402 --> ing1_Meat_extract
    ing2_Agar["Agar"]
    CultureMech_001402 --> ing2_Agar
    CHEBI_2509["CHEBI:2509"]
    ing2_Agar -.-> CHEBI_2509
    ing3_Horse_serum["Horse serum"]
    CultureMech_001402 --> ing3_Horse_serum

Ingredients (4)

# Ingredient Concentration Ontology Notes
1 Peptone 5 G_PER_L MICRO:0000178 (source link unavailable)
Peptone
2 Meat extract 3 G_PER_L
3 Agar 15 G_PER_L CHEBI:2509
agar
 (if necessary)
4 Horse serum 100 G_PER_L

Preparation

  1. Adjust pH to 7.0. For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation.
  2. To autoclaved medium add:

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:10.714227Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 302
2026-03-13T06:19:03.800011Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001402
2026-03-17T06:30:56.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:15.770488+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "NUTRIENT BROTH WITH 10% HORSE SERUM" to "nutrient_broth_with_10_horse_serum" for consistent matching
2026-06-05T07:41:40.192336+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-13T00:00:00-07:00 repair_bacterial_score10_exact_terms_batch7.py GROUNDED_BACTERIAL_SCORE10_EXACT_TERMS_BATCH7 Applied local mappings for Peptone.