simonsiella_medium

CultureMech:001403 bacterial COMPLEX SOLID_AGAR
Original name: SIMONSIELLA MEDIUM

Overview

External ontology
mediadive.medium:303 (source link unavailable) — SIMONSIELLA MEDIUM
Applications
  • Microbial cultivation
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium303.pdf

Composition

flowchart LR
    CultureMech_001403["simonsiella_medium"]
    ing0_Trypticase["Trypticase"]
    CultureMech_001403 --> ing0_Trypticase
    ing1_Phytone["Phytone"]
    CultureMech_001403 --> ing1_Phytone
    ing2_NaCl["NaCl"]
    CultureMech_001403 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_K2HPO4["K2HPO4"]
    CultureMech_001403 --> ing3_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing3_K2HPO4 -.-> CHEBI_131527
    ing4_Yeast_extract["Yeast extract"]
    CultureMech_001403 --> ing4_Yeast_extract
    ing5_Horse_serum["Horse serum"]
    CultureMech_001403 --> ing5_Horse_serum
    ing6_Agar["Agar"]
    CultureMech_001403 --> ing6_Agar
    CHEBI_2509["CHEBI:2509"]
    ing6_Agar -.-> CHEBI_2509

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 Trypticase 17 G_PER_L MICRO:0000175 (source link unavailable)
Trypticase peptone
2 Phytone 3 G_PER_L
3 NaCl
Cl.Na 58.443 g/mol
5 G_PER_L CHEBI:26710
sodium chloride
4 K2HPO4
HO4P.2K 174.174 g/mol
2.5 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
5 Yeast extract 4 G_PER_L
6 Horse serum 100 G_PER_L
7 Agar 15 G_PER_L CHEBI:2509
agar
 (if necessary)

Preparation

  1. Prepare the medium without horse serum. After autoclaving cool to 50°C, then add 100 ml/l of sterile horse serum. Transfer cultures when actively growing. Cultures die rapidly in stationary growth phase!

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:10.715304Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 303
2026-01-28T19:48:32.623660Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:03.803880Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001403
2026-03-17T06:30:34.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:15.774926+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "SIMONSIELLA MEDIUM" to "simonsiella_medium" for consistent matching
2026-06-05T07:41:45.855904+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:11.494868+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-09-22T00:03:26.686152+00:00 repair_rejected_peptone_groundings.py CORRECTED_REJECTED_PEPTONE_GROUNDING CultureMech #484. /ingredients/0/term/id: Trypticase CHEBI:78018 -> MICRO:0000175 (replace_term). Reviewed identities: https://github.com/CultureBotAI/MediaIngredientMech/tree/24562493919391df4468a4285b7c997960cc8868. Publication resolver: MIM label index 9f09e4fb97fb0e6cbbd6f25baca40b36512adb88. MediaDive source IDs and concentrations preserved.
2026-10-07T10:55:41.454416+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.