Overview
- External ontology
- mediadive.medium:309 (source link unavailable) — NEOMYCIN AGAR
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium309.pdf
Composition
flowchart LR
CultureMech_001407["neomycin_agar"]
ing0_Beef_extract["Beef extract"]
CultureMech_001407 --> ing0_Beef_extract
ing1_Yeast_extract["Yeast extract"]
CultureMech_001407 --> ing1_Yeast_extract
ing2_Peptone["Peptone"]
CultureMech_001407 --> ing2_Peptone
ing3_Glucose["Glucose"]
CultureMech_001407 --> ing3_Glucose
CHEBI_17234["CHEBI:17234"]
ing3_Glucose -.-> CHEBI_17234
ing4_Casitone["Casitone"]
CultureMech_001407 --> ing4_Casitone
ing5_Neomycin["Neomycin"]
CultureMech_001407 --> ing5_Neomycin
CHEBI_7507["CHEBI:7507"]
ing5_Neomycin -.-> CHEBI_7507
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Beef extract | 1.5 G_PER_L |
FOODON:03302088
Beef extract
|
|
| 2 | Yeast extract | 3 G_PER_L |
FOODON:03315426
Yeast extract
|
|
| 3 | Peptone | 6 G_PER_L |
MICRO:0000178 (source link unavailable)
Peptone
|
|
| 4 |
Glucose
C6H12O6 180.156 g/mol
|
1 G_PER_L |
CHEBI:17234
glucose
|
|
| 5 | Casitone | 4 G_PER_L |
MICRO:0000606 (source link unavailable)
Casitone
|
|
| 6 | Neomycin | 1.0 MG_PER_ML |
CHEBI:7507
neomycin
|
Selective agent; added aseptically to a final concentration of 1.0 mg/ml per preparation step 2. |
Preparation
- Adjust pH to 7.0.
- Aseptically add neomycin to a final concentration of 1.0 mg/ml of medium.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.720043Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 309 |
| 2026-03-13T06:19:03.816507Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001407 |
| 2026-03-17T06:30:37.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:15.789045+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "NEOMYCIN AGAR" to "neomycin_agar" for consistent matching |
| 2026-06-05T07:41:39.587605+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T05:34:58.042742+00:00 | curate_selective_agent.py | ADDED_SELECTIVE_AGENT | Added neomycin 1.0 mg/ml, recovered from the record's own preparation_steps / source duplicate (#181) |
| 2026-08-20T19:02:59.978214+00:00 | apply_mim_groundings.py | Adopted MIM published grounding | Grounded 1 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308). |
| 2026-09-13T00:00:00-07:00 | repair_bacterial_score10_exact_terms_batch6.py | GROUNDED_BACTERIAL_SCORE10_EXACT_TERMS_BATCH6 | Applied local mappings for Beef extract, Casitone, Peptone, Yeast extract. |