Overview
- External ontology
- mediadive.medium:31 (source link unavailable) — ALKALINE NUTRIENT AGAR
- Applications
-
- Microbial cultivation
- pH
- 9.7
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium31.pdf
Composition
flowchart LR
CultureMech_001421["alkaline_nutrient_agar"]
ing0_Peptone["Peptone"]
CultureMech_001421 --> ing0_Peptone
ing1_Meat_extract["Meat extract"]
CultureMech_001421 --> ing1_Meat_extract
ing2_Agar["Agar"]
CultureMech_001421 --> ing2_Agar
CHEBI_2509["CHEBI:2509"]
ing2_Agar -.-> CHEBI_2509
ing3_NaHCO3["NaHCO3"]
CultureMech_001421 --> ing3_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing3_NaHCO3 -.-> CHEBI_32139
ing4_Na2CO3_anhydrous["Na2CO3 anhydrous"]
CultureMech_001421 --> ing4_Na2CO3_anhydrous
CHEBI_29377["CHEBI:29377"]
ing4_Na2CO3_anhydrous -.-> CHEBI_29377
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Peptone | 5 G_PER_L | ||
| 2 | Meat extract | 3 G_PER_L | ||
| 3 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
(if necessary) |
| 4 |
NaHCO3
CHO3.Na 84.006 g/mol
|
42 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 5 |
Na2CO3 anhydrous
CO3.2Na 105.988 g/mol
|
53 G_PER_L |
CHEBI:29377
sodium carbonate
|
Preparation
- For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation.
- After sterilization add sterile 1 M Na-sesquicarbonate solution (1 ml in 10 ml) to achieve a pH of 9.7
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.014257Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 31 |
| 2026-03-13T06:19:03.988780Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001421 |
| 2026-03-17T06:30:41.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:15.971318+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ALKALINE NUTRIENT AGAR" to "alkaline_nutrient_agar" for consistent matching |
| 2026-06-05T07:40:47.898909+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |