methanocorpusculum_aggregans_medium (CultureMech:001423)

Overview

External ontology

mediadive.medium:321 (source link unavailable) — METHANOCORPUSCULUM AGGREGANS MEDIUM

Applications

Microbial cultivation

pH range

{'min': 6.8, 'max': 7.0}

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium321.pdf

Composition

flowchart LR
    CultureMech_001423["methanocorpusculum_aggregans_medium"]
    ing0_NH4Cl["NH4Cl"]
    CultureMech_001423 --> ing0_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing0_NH4Cl -.-> CHEBI_31206
    ing1_K2HPO4_x_3_H2O["K2HPO4 x 3 H2O"]
    CultureMech_001423 --> ing1_K2HPO4_x_3_H2O
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4_x_3_H2O -.-> CHEBI_131527
    ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001423 --> ing2_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing3_Na_formate["Na-formate"]
    CultureMech_001423 --> ing3_Na_formate
    CHEBI_62965["CHEBI:62965"]
    ing3_Na_formate -.-> CHEBI_62965
    ing4_Na_acetate["Na-acetate"]
    CultureMech_001423 --> ing4_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing4_Na_acetate -.-> CHEBI_32954
    ing5_Trypticase_peptone["Trypticase peptone"]
    CultureMech_001423 --> ing5_Trypticase_peptone
    ing6_Yeast_extract["Yeast extract"]
    CultureMech_001423 --> ing6_Yeast_extract
    ing7_Sodium_resazurin["Sodium resazurin"]
    CultureMech_001423 --> ing7_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing7_Sodium_resazurin -.-> CHEBI_8806
    ing8_Na2CO3["Na2CO3"]
    CultureMech_001423 --> ing8_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing8_Na2CO3 -.-> CHEBI_29377
    ing9_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_001423 --> ing9_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing9_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing10_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_001423 --> ing10_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing10_Na2S_x_9_H2O -.-> CHEBI_76209
    ing11_KH2PO4["KH2PO4"]
    CultureMech_001423 --> ing11_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing11_KH2PO4 -.-> CHEBI_63036
    ing12__NH4_2SO4["(NH4)2SO4"]
    CultureMech_001423 --> ing12__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing12__NH4_2SO4 -.-> CHEBI_62946
    ing13_NaCl["NaCl"]
    CultureMech_001423 --> ing13_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing13_NaCl -.-> CHEBI_26710
    ing14_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001423 --> ing14_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing14_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing15_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001423 --> ing15_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing15_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing16_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_001423 --> ing16_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing16_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing17_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_001423 --> ing17_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing17_MnSO4_x_H2O -.-> CHEBI_86364
    ing18_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001423 --> ing18_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing18_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing19_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_001423 --> ing19_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing19_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing20_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001423 --> ing20_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing20_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing21_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001423 --> ing21_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing21_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing22_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_001423 --> ing22_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing22_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing23_H3BO3["H3BO3"]
    CultureMech_001423 --> ing23_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing23_H3BO3 -.-> CHEBI_33118
    ing24_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001423 --> ing24_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing24_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing25_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001423 --> ing25_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing25_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing26_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_001423 --> ing26_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing26_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing27_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_001423 --> ing27_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing27_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing28_Sludge["Sludge"]
    CultureMech_001423 --> ing28_Sludge

Ingredients (29)

#	Ingredient	Concentration	Ontology	Notes
1	NH4Cl Cl.H4N 53.492 g/mol	1 G_PER_L	CHEBI:31206 ammonium chloride
2	K2HPO4 x 3 H2O HO4P.2K 174.174 g/mol	0.4 G_PER_L	CHEBI:131527 dipotassium hydrogen phosphate
3	MgCl2 x 6 H2O 2Cl.6H2O.Mg 203.301 g/mol	0.4 G_PER_L	CHEBI:86345 magnesium dichloride hexahydrate
4	Na-formate CHO2.Na 68.007 g/mol	5 G_PER_L	CHEBI:62965 sodium formate
5	Na-acetate C2H3O2.Na 82.034 g/mol	1 G_PER_L	CHEBI:32954 sodium acetate
6	Trypticase peptone	1 G_PER_L
7	Yeast extract	5.0 G_PER_L		[Merged 2 duplicates: 1.0, 4.0]
8	Sodium resazurin C12H7NO4 229.191 g/mol	0.0005 G_PER_L	CHEBI:8806 Resazurin
9	Na2CO3 CO3.2Na 105.988 g/mol	1.5 G_PER_L	CHEBI:29377 sodium carbonate
10	L-Cysteine HCl x H2O C3H7NO2S.H2O.HCl 175.637 g/mol	0.2 G_PER_L	CHEBI:91248 L-cysteine hydrochloride hydrate
11	Na2S x 9 H2O 9H2O.2Na.S 240.182 g/mol	0.2 G_PER_L	CHEBI:76209 sodium sulfide nonahydrate
12	KH2PO4 H2O4P.K 136.084 g/mol	6 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate
13	(NH4)2SO4 2H4N.O4S 132.141 g/mol	6 G_PER_L	CHEBI:62946 ammonium sulfate
14	NaCl Cl.Na 58.443 g/mol	13.0 G_PER_L	CHEBI:26710 sodium chloride	[Merged 2 duplicates: 12.0, 1.0]
15	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol	5.6 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate	[Merged 2 duplicates: 2.6, 3.0]
16	CaCl2 x 2 H2O Ca.2Cl.2H2O 147.014 g/mol	0.26 G_PER_L	CHEBI:86158 calcium chloride dihydrate	[Merged 2 duplicates: 0.16, 0.1]
17	Nitrilotriacetic acid C6H9NO6 191.139 g/mol	1.5 G_PER_L	CHEBI:44557 nitrilotriacetic acid
18	MnSO4 x H2O H2O.Mn.O4S 169.016 g/mol	0.5 G_PER_L	CHEBI:86364 manganese(II) sulfate monohydrate
19	FeSO4 x 7 H2O Fe.7H2O.O4S 278.013 g/mol	0.1 G_PER_L	CHEBI:75836 iron(2+) sulfate heptahydrate
20	CoSO4 x 7 H2O Co.7H2O.O4S 281.101 g/mol	0.18 G_PER_L	CHEBI:91244 cobalt(2+) sulfate heptahydrate
21	ZnSO4 x 7 H2O 7H2O.O4S.Zn 287.558 g/mol	0.18 G_PER_L	CHEBI:32312 zinc sulfate heptahydrate
22	CuSO4 x 5 H2O Cu.5H2O.O4S 249.684 g/mol	0.01 G_PER_L	CHEBI:31440 copper(II) sulfate pentahydrate
23	AlK(SO4)2 x 12 H2O Al.12H2O.K.2O4S 474.386 g/mol	0.02 G_PER_L	CHEBI:86465 potassium aluminium sulfate dodecahydrate
24	H3BO3 H3BO3 61.833 g/mol	0.01 G_PER_L	CHEBI:33118 boric acid
25	Na2MoO4 x 2 H2O 2H2O.MoO4.2Na 241.946 g/mol	0.01 G_PER_L	CHEBI:75213 sodium molybdate dihydrate
26	NiCl2 x 6 H2O Cl2Ni 129.599 g/mol	0.03 G_PER_L	CHEBI:34887 nickel dichloride
27	Na2SeO3 x 5 H2O 5H2O.2Na.O3Se 263.012 g/mol	0.0003 G_PER_L	CHEBI:131361 disodium selenite pentahydrate
28	Na2WO4 x 2 H2O 2H2O.2Na.O4W 329.846 g/mol	0.0004 G_PER_L	CHEBI:63939 sodium tungstate dihydrate
29	Sludge	1000 G_PER_L

Preparation

Dissolve ingredients except carbonate, cysteine and sulfide, then sparge medium with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% of their volume and autoclave. Add cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use check pH of complete medium and adjust to 6.8 - 7.0, if necessary.
After inoculation add sterile 80% H2 and 20% CO2 gas mixture to 2 bar overpressure.
First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
Rumen fluid from cow or sheep (obtained from fistulated animals or abattoir refuse) is filtered through muslin, autoclaved at 121°C for 15 min and then centrifuged at 27,000 g for 20 min. The supernatant is made anoxic by sparging with 100% N2 gas for 15 min, dispensed under same gas atmosphere into anoxic serum vials to 30% of volume and then stored frozen at -20°C.
Add 0.4% yeast extract to sludge from an anaerobic digester, and after gassing with nitrogen gas for a few minutes incubate it at 37°C for 24 hours. Then centrifuge the sludge at 13000 g and autoclave the resulting, clear supernatant in screw-capped vessels under nitrogen gas. The sludge fluid can be stored at 8-12°C in the dark.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-26T17:53:10.786858Z	mediadive-import	Imported from MediaDive	Source: DSMZ, ID: 321
2026-03-13T03:09:41.221381Z	data-quality-cleanup-v1.0	Data quality fixes	Merged 4 duplicate ingredient(s)
2026-03-13T06:19:04.012814Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:001423
2026-03-17T06:30:53.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:15.995466+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "METHANOCORPUSCULUM AGGREGANS MEDIUM" to "methanocorpusculum_aggregans_medium" for consistent matching
2026-05-20T06:01:55.518076+00:00	migrate_ph_range.py	MIGRATED_PH_RANGE	ph_range '6.8-7' -> {'min': 6.8, 'max': 7.0}
2026-06-05T07:41:31.321214+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 25 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:06.897437+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	8 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.