rumen_bacteria_medium

Overview

External ontology

mediadive.medium:330 (source link unavailable) — RUMEN BACTERIA MEDIUM

Applications

• Microbial cultivation

pH range

{'min': 6.7, 'max': 6.8}

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium330.pdf Superseded historical provenance, not a current formulation claim: Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification. The source explicitly states nominal final volume 1003 ml. Listed liquid inputs total 1003.6 ml; do not substitute that sum, assume volume additivity, or claim measured precision. Main mass rates use the stated 1.003 L denominator, rounded to six significant digits. Stock batch masses without a final-volume instruction remain in notes. Preparation stages and the strain-specific 30 ml/l rumen-fluid supplement remain source context, not a base ingredient or new growth association. Preserve CO2 treatment and post-autoclave sterile anoxic additions. Complete inspected source rows define this reconstruction. Archive old flattened quantities, false LB expansions, placeholders and copied stock-solvent claims in append-only history. Unrelated annotations remain preserved, not recertified.

Composition

flowchart LR
    CultureMech_001429["rumen_bacteria_medium"]
    ing0_K2HPO4["K2HPO4"]
    CultureMech_001429 --> ing0_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing0_K2HPO4 -.-> CHEBI_131527
    ing1_Trypticase_peptone__BD_BBL_["Trypticase peptone (BD BBL)"]
    CultureMech_001429 --> ing1_Trypticase_peptone__BD_BBL_
    ing2_Yeast_extract__OXOID_["Yeast extract (OXOID)"]
    CultureMech_001429 --> ing2_Yeast_extract__OXOID_
    ing3_Glycerol["Glycerol"]
    CultureMech_001429 --> ing3_Glycerol
    CHEBI_17754["CHEBI:17754"]
    ing3_Glycerol -.-> CHEBI_17754
    ing4_Sodium_resazurin__0_1__w_v_["Sodium resazurin (0.1% w/v)"]
    CultureMech_001429 --> ing4_Sodium_resazurin__0_1__w_v_
    ing5_Na2CO3["Na2CO3"]
    CultureMech_001429 --> ing5_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing5_Na2CO3 -.-> CHEBI_29377
    ing6_D_Glucose["D-Glucose"]
    CultureMech_001429 --> ing6_D_Glucose
    CHEBI_17634["CHEBI:17634"]
    ing6_D_Glucose -.-> CHEBI_17634
    ing7_Maltose["Maltose"]
    CultureMech_001429 --> ing7_Maltose
    CHEBI_17306["CHEBI:17306"]
    ing7_Maltose -.-> CHEBI_17306
    ing8_Cellobiose["Cellobiose"]
    CultureMech_001429 --> ing8_Cellobiose
    CHEBI_17057["CHEBI:17057"]
    ing8_Cellobiose -.-> CHEBI_17057
    ing9_Starch__soluble["Starch, soluble"]
    CultureMech_001429 --> ing9_Starch__soluble
    CHEBI_28017["CHEBI:28017"]
    ing9_Starch__soluble -.-> CHEBI_28017
    ing10_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_001429 --> ing10_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing10_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing11_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_001429 --> ing11_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing11_Na2S_x_9_H2O -.-> CHEBI_76209
    ing12_Distilled_water["Distilled water"]
    CultureMech_001429 --> ing12_Distilled_water

Ingredients (13)

Preparation

1. Dissolve ingredients (except carbonate, glucose, maltose, cellobiose, soluble starch, cysteine and sulfide), then sparge medium with 100% CO2 gas for 30 - 45 min to make it anoxic. Add the carbonate and equilibrate the medium with the CO2 gas to pH 6.8. Distribute under 100% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Thereafter, add glucose, maltose, cellobiose, soluble starch, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere. Adjust pH of complete medium to 6.7 - 6.8, if necessary.
2. Dissolve 50 mg haemin in 1 ml 1 N NaOH; make up to 100 ml with distilled water and filter sterilize. Store refrigerated.

Solutions

Target organisms

No target organisms are recorded.

Provenance