methanoculleus_bourgensis_medium

CultureMech:001431 archaea COMPLEX LIQUID
Original name: METHANOCULLEUS BOURGENSIS MEDIUM

Overview

External ontology
mediadive.medium:332 (source link unavailable) — METHANOCULLEUS BOURGENSIS MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 6.8, 'max': 7.0}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium332.pdf

Composition

flowchart LR
    CultureMech_001431["methanoculleus_bourgensis_medium"]
    ing0_NH4Cl["NH4Cl"]
    CultureMech_001431 --> ing0_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing0_NH4Cl -.-> CHEBI_31206
    ing1_K2HPO4_x_3_H2O["K2HPO4 x 3 H2O"]
    CultureMech_001431 --> ing1_K2HPO4_x_3_H2O
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4_x_3_H2O -.-> CHEBI_131527
    ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001431 --> ing2_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing3_Na_formate["Na-formate"]
    CultureMech_001431 --> ing3_Na_formate
    CHEBI_62965["CHEBI:62965"]
    ing3_Na_formate -.-> CHEBI_62965
    ing4_Na_acetate["Na-acetate"]
    CultureMech_001431 --> ing4_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing4_Na_acetate -.-> CHEBI_32954
    ing5_Trypticase_peptone["Trypticase peptone"]
    CultureMech_001431 --> ing5_Trypticase_peptone
    ing6_Yeast_extract["Yeast extract"]
    CultureMech_001431 --> ing6_Yeast_extract
    ing7_Sodium_resazurin["Sodium resazurin"]
    CultureMech_001431 --> ing7_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing7_Sodium_resazurin -.-> CHEBI_8806
    ing8_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_001431 --> ing8_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing8_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing9_Na2CO3["Na2CO3"]
    CultureMech_001431 --> ing9_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing9_Na2CO3 -.-> CHEBI_29377
    ing10_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_001431 --> ing10_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing10_Na2S_x_9_H2O -.-> CHEBI_76209

Ingredients (11)

# Ingredient Concentration Ontology Notes
1 NH4Cl
Cl.H4N 53.492 g/mol
1 G_PER_L CHEBI:31206
ammonium chloride
2 K2HPO4 x 3 H2O
HO4P.2K 174.174 g/mol
0.4 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
3 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.1 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
4 Na-formate
CHO2.Na 68.007 g/mol
5 G_PER_L CHEBI:62965
sodium formate
5 Na-acetate
C2H3O2.Na 82.034 g/mol
1 G_PER_L CHEBI:32954
sodium acetate
6 Trypticase peptone 1 G_PER_L
7 Yeast extract 1 G_PER_L
8 Sodium resazurin
C12H7NO4 229.191 g/mol
0.0005 G_PER_L CHEBI:8806
Resazurin
9 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.5 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
10 Na2CO3
CO3.2Na 105.988 g/mol
1.5 G_PER_L CHEBI:29377
sodium carbonate
11 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.2 G_PER_L CHEBI:76209
sodium sulfide nonahydrate

Preparation

  1. Dissolve ingredients except cysteine, carbonate and sulfide. Sparge medium with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add and dissolve cysteine, then dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% of their volume and autoclave. Add carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture and sulfide from a sterile anoxic stock solution prepared under 100% N2 gas. Prior to use check pH of complete medium and adjust to 6.8 - 7.0, if necessary.
  2. Note: After growth has started add sterile 80% H2 and 20% CO2 gas mixture to 0.5 - 1 bar overpressure.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:10.823165Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 332
2026-03-13T06:19:04.117982Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001431
2026-03-17T06:30:20.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:16.108070+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "METHANOCULLEUS BOURGENSIS MEDIUM" to "methanoculleus_bourgensis_medium" for consistent matching
2026-05-20T06:01:55.536695+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '6.8-7' -> {'min': 6.8, 'max': 7.0}
2026-06-05T07:41:31.342480+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:06.907917+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T10:55:41.454416+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.