Overview
- External ontology
- mediadive.medium:338 (source link unavailable) — CAMPYLOBACTER RECTUS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.5
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium338.pdf
Composition
flowchart LR
CultureMech_001438["campylobacter_rectus_medium"]
ing0_Beef_extract["Beef extract"]
CultureMech_001438 --> ing0_Beef_extract
ing1_Trypticase["Trypticase"]
CultureMech_001438 --> ing1_Trypticase
ing2_Yeast_extract["Yeast extract"]
CultureMech_001438 --> ing2_Yeast_extract
ing3_NaCl["NaCl"]
CultureMech_001438 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_Na2HPO4["Na2HPO4"]
CultureMech_001438 --> ing4_Na2HPO4
CHEBI_34683["CHEBI:34683"]
ing4_Na2HPO4 -.-> CHEBI_34683
ing5_Na2CO3["Na2CO3"]
CultureMech_001438 --> ing5_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing5_Na2CO3 -.-> CHEBI_29377
ing6_Haemin["Haemin"]
CultureMech_001438 --> ing6_Haemin
CHEBI_50385["CHEBI:50385"]
ing6_Haemin -.-> CHEBI_50385
ing7_Na_formate["Na-formate"]
CultureMech_001438 --> ing7_Na_formate
CHEBI_62965["CHEBI:62965"]
ing7_Na_formate -.-> CHEBI_62965
ing8_Na_fumarate["Na-fumarate"]
CultureMech_001438 --> ing8_Na_fumarate
CHEBI_115156["CHEBI:115156"]
ing8_Na_fumarate -.-> CHEBI_115156
ing9_Resazurin["Resazurin"]
CultureMech_001438 --> ing9_Resazurin
CHEBI_8806["CHEBI:8806"]
ing9_Resazurin -.-> CHEBI_8806
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Beef extract | 3 G_PER_L | ||
| 2 | Trypticase | 9 G_PER_L |
MICRO:0000175 (source link unavailable)
Trypticase peptone
|
|
| 3 | Yeast extract | 11 G_PER_L | ||
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
2 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 5 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
0.4 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 6 |
Na2CO3
CO3.2Na 105.988 g/mol
|
0.25 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 7 |
Haemin
C34H32ClFeN4O4 651.952 g/mol
|
0.005 G_PER_L |
CHEBI:50385
hemin
|
|
| 8 |
Na-formate
CHO2.Na 68.007 g/mol
|
2 G_PER_L |
CHEBI:62965
sodium formate
|
|
| 9 |
Na-fumarate
C4H2O4.2Na 160.036 g/mol
|
3 G_PER_L |
CHEBI:115156
disodium fumarate
|
|
| 10 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.001 G_PER_L |
CHEBI:8806
Resazurin
|
Preparation
- Adjust pH to 7.5. Prepare the medium anaerobically under 100% N2.
- Autoclave solutions of haemin (dissolved in 0.1 ml 1N NaOH, then diluted with water to 10 ml and neutralized), formate, and fumarate separately under nitrogen.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.849826Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 338 |
| 2026-01-28T19:48:32.084268Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:04.203193Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001438 |
| 2026-03-17T06:30:33.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:16.200974+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CAMPYLOBACTER RECTUS MEDIUM" to "campylobacter_rectus_medium" for consistent matching |
| 2026-06-05T07:40:52.478554+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:54.286326+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-22T00:03:26.686152+00:00 | repair_rejected_peptone_groundings.py | CORRECTED_REJECTED_PEPTONE_GROUNDING | CultureMech #484. /ingredients/1/term/id: Trypticase CHEBI:78018 -> MICRO:0000175 (replace_term). Reviewed identities: https://github.com/CultureBotAI/MediaIngredientMech/tree/24562493919391df4468a4285b7c997960cc8868. Publication resolver: MIM label index 9f09e4fb97fb0e6cbbd6f25baca40b36512adb88. MediaDive source IDs and concentrations preserved. |
| 2026-10-07T10:55:41.454416+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |