Overview
- External ontology
- mediadive.medium:34 (source link unavailable) — CARYOPHANON LATUM MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.8
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium34.pdf
Composition
flowchart LR
CultureMech_001449["caryophanon_latum_medium"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_001449 --> ing0_Yeast_extract
ing1_Trypticase["Trypticase"]
CultureMech_001449 --> ing1_Trypticase
ing2_Soy_peptone["Soy peptone"]
CultureMech_001449 --> ing2_Soy_peptone
ing3_Na_acetate["Na-acetate"]
CultureMech_001449 --> ing3_Na_acetate
CHEBI_32954["CHEBI:32954"]
ing3_Na_acetate -.-> CHEBI_32954
ing4_Na_glutamate["Na glutamate"]
CultureMech_001449 --> ing4_Na_glutamate
CHEBI_64220["CHEBI:64220"]
ing4_Na_glutamate -.-> CHEBI_64220
ing5_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_001449 --> ing5_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing5_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
ing6_Biotin["Biotin"]
CultureMech_001449 --> ing6_Biotin
CHEBI_15956["CHEBI:15956"]
ing6_Biotin -.-> CHEBI_15956
ing7_K2HPO4["K2HPO4"]
CultureMech_001449 --> ing7_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing7_K2HPO4 -.-> CHEBI_131527
ing8_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001449 --> ing8_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing8_MgSO4_x_7_H2O -.-> CHEBI_31795
ing9_Tris_HCl_buffer["Tris-HCl buffer"]
CultureMech_001449 --> ing9_Tris_HCl_buffer
CHEBI_9754["CHEBI:9754"]
ing9_Tris_HCl_buffer -.-> CHEBI_9754
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 2 G_PER_L | ||
| 2 | Trypticase | 2 G_PER_L |
MICRO:0000175 (source link unavailable)
Trypticase peptone
|
|
| 3 | Soy peptone | 2 G_PER_L | ||
| 4 |
Na-acetate
C2H3O2.Na 82.034 g/mol
|
1 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 5 |
Na glutamate
C5H8NO4.Na 169.112 g/mol
|
0.1 G_PER_L |
CHEBI:64220
monosodium glutamate
|
|
| 6 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.0002 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
|
| 7 |
Biotin
C10H16N2O3S 244.316 g/mol
|
5e-05 G_PER_L |
CHEBI:15956
biotin
|
|
| 8 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 9 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.27 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 10 |
Tris-HCl buffer
C4H11NO3 121.136 g/mol
|
1000 G_PER_L |
CHEBI:9754
tris
|
(pH 7.8) |
Preparation
- Adjust pH to 7.8.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.017078Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 34 |
| 2026-01-28T19:48:19.978534Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:04.310503Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001449 |
| 2026-03-17T06:30:08.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:16.317683+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CARYOPHANON LATUM MEDIUM" to "caryophanon_latum_medium" for consistent matching |
| 2026-06-05T07:40:52.663388+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:54.355119+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-22T00:03:26.686152+00:00 | repair_rejected_peptone_groundings.py | CORRECTED_REJECTED_PEPTONE_GROUNDING | CultureMech #484. /ingredients/1/term/id: Trypticase CHEBI:78018 -> MICRO:0000175 (replace_term). Reviewed identities: https://github.com/CultureBotAI/MediaIngredientMech/tree/24562493919391df4468a4285b7c997960cc8868. Publication resolver: MIM label index 9f09e4fb97fb0e6cbbd6f25baca40b36512adb88. MediaDive source IDs and concentrations preserved. |
| 2026-10-07T10:55:41.454416+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |