Overview
- External ontology
- mediadive.medium:350 (source link unavailable) — CELLULOMONAS FERMENTANS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.4
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium350.pdf
Composition
flowchart LR
CultureMech_001450["cellulomonas_fermentans_medium"]
ing0_K2HPO4["K2HPO4"]
CultureMech_001450 --> ing0_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing0_K2HPO4 -.-> CHEBI_131527
ing1_KH2PO4["KH2PO4"]
CultureMech_001450 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2__NH4_2SO4["(NH4)2SO4"]
CultureMech_001450 --> ing2__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing2__NH4_2SO4 -.-> CHEBI_62946
ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001450 --> ing3_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001450 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001450 --> ing5_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing5_FeSO4_x_7_H2O -.-> CHEBI_75836
ing6_Yeast_extract["Yeast extract"]
CultureMech_001450 --> ing6_Yeast_extract
ing7_Cellobiose["Cellobiose"]
CultureMech_001450 --> ing7_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing7_Cellobiose -.-> CHEBI_17057
ing8_NaHCO3["NaHCO3"]
CultureMech_001450 --> ing8_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing8_NaHCO3 -.-> CHEBI_32139
ing9_Resazurin["Resazurin"]
CultureMech_001450 --> ing9_Resazurin
CHEBI_8806["CHEBI:8806"]
ing9_Resazurin -.-> CHEBI_8806
ing10_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_001450 --> ing10_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing10_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
K2HPO4
HO4P.2K 174.174 g/mol
|
2.21 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1.3 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 4 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.1 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.02 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 6 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.00125 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 7 | Yeast extract | 5 G_PER_L | ||
| 8 |
Cellobiose
C12H22O11 342.297 g/mol
|
5 G_PER_L |
CHEBI:17057
cellobiose
|
|
| 9 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.8 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 10 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.001 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 11 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Preparation
- Adjust pH to 7.4 with 8 M NaOH.
- Prepare the medium anaerobically under 100% nitrogen. Cellobiose, NaHCO3, and cysteine are sterilized separately by autoclaving under N2.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.886735Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 350 |
| 2026-03-13T06:19:04.316208Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001450 |
| 2026-03-17T06:30:04.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:16.324271+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CELLULOMONAS FERMENTANS MEDIUM" to "cellulomonas_fermentans_medium" for consistent matching |
| 2026-06-05T07:40:52.936253+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:54.419543+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-07T10:55:41.454416+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |