Overview
- External ontology
- mediadive.medium:352 (source link unavailable) — AZOSPIRILLUM AMAZONENSE MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium352.pdf
Composition
flowchart LR
CultureMech_001452["azospirillum_amazonense_medium"]
ing0_K2HPO4["K2HPO4"]
CultureMech_001452 --> ing0_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing0_K2HPO4 -.-> CHEBI_131527
ing1_KH2PO4["KH2PO4"]
CultureMech_001452 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001452 --> ing2_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001452 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001452 --> ing4_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing4_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing5_FeCl3["FeCl3"]
CultureMech_001452 --> ing5_FeCl3
CHEBI_30808["CHEBI:30808"]
ing5_FeCl3 -.-> CHEBI_30808
ing6_Bromothymol_blue["Bromothymol blue"]
CultureMech_001452 --> ing6_Bromothymol_blue
CHEBI_86155["CHEBI:86155"]
ing6_Bromothymol_blue -.-> CHEBI_86155
ing7_Sucrose["Sucrose"]
CultureMech_001452 --> ing7_Sucrose
CHEBI_17992["CHEBI:17992"]
ing7_Sucrose -.-> CHEBI_17992
ing8_Agar["Agar"]
CultureMech_001452 --> ing8_Agar
CHEBI_2509["CHEBI:2509"]
ing8_Agar -.-> CHEBI_2509
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.2 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.6 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.02 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.002 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 6 |
FeCl3
Cl3Fe 162.204 g/mol
|
0.01 G_PER_L |
CHEBI:30808
iron trichloride
|
|
| 7 |
Bromothymol blue
C27H28Br2O5S 624.391 g/mol
|
0.025 G_PER_L |
CHEBI:86155
bromothymol blue
|
|
| 8 |
Sucrose
C12H22O11 342.297 g/mol
|
5 G_PER_L |
CHEBI:17992
sucrose
|
|
| 9 | Agar | 1.75 G_PER_L |
CHEBI:2509
agar
|
Preparation
- Adjust pH to 6.0.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.892680Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 352 |
| 2026-03-13T06:19:04.334401Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001452 |
| 2026-03-17T06:30:12.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:16.344429+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "AZOSPIRILLUM AMAZONENSE MEDIUM" to "azospirillum_amazonense_medium" for consistent matching |
| 2026-06-05T07:40:50.155238+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:53.673215+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-07T10:55:41.454416+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |