l_wenstein_jensen_medium

CultureMech:001454 bacterial COMPLEX LIQUID
Original name: LÖWENSTEIN-JENSEN MEDIUM

Overview

External ontology
mediadive.medium:354 (source link unavailable) — LÖWENSTEIN-JENSEN MEDIUM
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium354.pdf

Composition

flowchart LR
    CultureMech_001454["l_wenstein_jensen_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_001454 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_MgSO4["MgSO4"]
    CultureMech_001454 --> ing1_MgSO4
    CHEBI_32599["CHEBI:32599"]
    ing1_MgSO4 -.-> CHEBI_32599
    ing2_Mg_citrate["Mg-citrate"]
    CultureMech_001454 --> ing2_Mg_citrate
    CHEBI_131389["CHEBI:131389"]
    ing2_Mg_citrate -.-> CHEBI_131389
    ing3_L_Asparagin["L-Asparagin"]
    CultureMech_001454 --> ing3_L_Asparagin
    CHEBI_17196["CHEBI:17196"]
    ing3_L_Asparagin -.-> CHEBI_17196
    ing4_Potato_flour["Potato flour"]
    CultureMech_001454 --> ing4_Potato_flour
    ing5_Malachite_green["Malachite green"]
    CultureMech_001454 --> ing5_Malachite_green
    CHEBI_72449["CHEBI:72449"]
    ing5_Malachite_green -.-> CHEBI_72449
    ing6_Glycerol["Glycerol"]
    CultureMech_001454 --> ing6_Glycerol
    CHEBI_17754["CHEBI:17754"]
    ing6_Glycerol -.-> CHEBI_17754
    ing7_Fresh_egg_mixture["Fresh egg mixture"]
    CultureMech_001454 --> ing7_Fresh_egg_mixture

Ingredients (8)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
1.55087 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 MgSO4
Mg.O4S 120.368 g/mol
0.148883 G_PER_L CHEBI:32599
magnesium sulfate
3 Mg-citrate
C6H6O7.Mg 214.412 g/mol
0.372208 G_PER_L CHEBI:131389
magnesium citrate
4 L-Asparagin
C4H8N2O3 132.119 g/mol
2.23325 G_PER_L CHEBI:17196
L-asparagine
5 Potato flour 18.6104 G_PER_L
6 Malachite green
C23H25N2.Cl 364.92 g/mol
0.248139 G_PER_L CHEBI:72449
malachite green
7 Glycerol
C3H8O3 92.094 g/mol
12 G_PER_L CHEBI:17754
glycerol
8 Fresh egg mixture 1000 G_PER_L

Preparation

  1. Addition:
  2. pH 7.0
  3. Sterilize medium for 30 min at 121°C, cool to 30°C and add fresh egg mixture carefully to the medium.
  4. * You need for 1 l of egg mixture 20 - 25 fresh eggs (not older then 1 week). Clean the eggs, wipe away with 70% alcohol, open the eggs and homogenize the egg mixture carefully.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:10.895484Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 354
2026-03-13T06:19:04.342310Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001454
2026-03-17T06:29:41.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:16.352560+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "LÖWENSTEIN-JENSEN MEDIUM" to "l_wenstein_jensen_medium" for consistent matching
2026-06-05T07:41:10.619348+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:00.443205+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T10:55:41.454416+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.