Overview
- External ontology
- mediadive.medium:366 (source link unavailable) — NAG MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium366.pdf
Composition
flowchart LR
CultureMech_001467["nag_medium"]
ing0_Peptone["Peptone"]
CultureMech_001467 --> ing0_Peptone
ing1_Meat_extract["Meat extract"]
CultureMech_001467 --> ing1_Meat_extract
ing2_Agar["Agar"]
CultureMech_001467 --> ing2_Agar
CHEBI_2509["CHEBI:2509"]
ing2_Agar -.-> CHEBI_2509
ing3_Glucose["Glucose"]
CultureMech_001467 --> ing3_Glucose
CHEBI_17234["CHEBI:17234"]
ing3_Glucose -.-> CHEBI_17234
Ingredients (4)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Peptone | 5 G_PER_L | ||
| 2 | Meat extract | 3 G_PER_L | ||
| 3 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
(if necessary) |
| 4 |
Glucose
C6H12O6 180.156 g/mol
|
10 G_PER_L |
CHEBI:17234
glucose
|
Preparation
- Adjust pH to 7.0. For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.916288Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 366 |
| 2026-03-13T06:19:04.405987Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001467 |
| 2026-03-17T06:30:17.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:16.424512+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "NAG MEDIUM" to "nag_medium" for consistent matching |
| 2026-06-05T07:41:39.136261+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-10-07T10:55:41.454416+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |