Overview
- External ontology
- mediadive.medium:368 (source link unavailable) — KUNKEE MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 5.5
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium368.pdf
Composition
flowchart LR
CultureMech_001469["kunkee_medium"]
ing0_Tryptone["Tryptone"]
CultureMech_001469 --> ing0_Tryptone
ing1_Peptone["Peptone"]
CultureMech_001469 --> ing1_Peptone
ing2_Yeast_extract["Yeast extract"]
CultureMech_001469 --> ing2_Yeast_extract
ing3_Glucose["Glucose"]
CultureMech_001469 --> ing3_Glucose
CHEBI_17234["CHEBI:17234"]
ing3_Glucose -.-> CHEBI_17234
ing4_Tween_80["Tween 80"]
CultureMech_001469 --> ing4_Tween_80
CHEBI_53426["CHEBI:53426"]
ing4_Tween_80 -.-> CHEBI_53426
ing5_Tomato_juice["Tomato juice"]
CultureMech_001469 --> ing5_Tomato_juice
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Tryptone | 20 G_PER_L |
MICRO:0000182 (source link unavailable)
Tryptone
|
|
| 2 | Peptone | 5 G_PER_L |
MICRO:0000178 (source link unavailable)
Peptone
|
|
| 3 | Yeast extract | 5 G_PER_L |
FOODON:03315426
Yeast extract
|
|
| 4 |
Glucose
C6H12O6 180.156 g/mol
|
5 G_PER_L |
CHEBI:17234
glucose
|
|
| 5 |
Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
|
0.5 G_PER_L |
CHEBI:53426
polysorbate 80
|
|
| 6 | Tomato juice | 250 G_PER_L |
FOODON:03301454
Tomato juice
|
Preparation
- Adjust pH to 5.5.
- After sterilization aseptically add 10% (v/v) ethanol
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.918715Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 368 |
| 2026-03-13T06:19:04.412128Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001469 |
| 2026-03-17T06:30:33.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:16.431292+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "KUNKEE MEDIUM" to "kunkee_medium" for consistent matching |
| 2026-06-05T07:41:10.590097+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-13T00:00:00-07:00 | repair_bacterial_score10_exact_terms_batch3.py | GROUNDED_BACTERIAL_SCORE10_EXACT_TERMS_BATCH3 | Applied local mappings for Peptone, Tomato juice, Tryptone, Yeast extract. |
| 2026-10-07T10:55:41.454416+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |