heliobacterium_chlorum_medium

CultureMech:001472 bacterial COMPLEX LIQUID
Original name: HELIOBACTERIUM CHLORUM MEDIUM

Overview

External ontology
mediadive.medium:370 (source link unavailable) — HELIOBACTERIUM CHLORUM MEDIUM
Applications
  • Microbial cultivation
pH
6.8
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium370.pdf

Composition

flowchart LR
    CultureMech_001472["heliobacterium_chlorum_medium"]
    ing0_K2HPO4["K2HPO4"]
    CultureMech_001472 --> ing0_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing0_K2HPO4 -.-> CHEBI_131527
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001472 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_001472 --> ing2_Yeast_extract
    ing3_Sodium_ascorbate["Sodium ascorbate"]
    CultureMech_001472 --> ing3_Sodium_ascorbate
    CHEBI_113451["CHEBI:113451"]
    ing3_Sodium_ascorbate -.-> CHEBI_113451

Ingredients (4)

# Ingredient Concentration Ontology Notes
1 K2HPO4
HO4P.2K 174.174 g/mol
1 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
2 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
1 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
3 Yeast extract 10 G_PER_L
4 Sodium ascorbate
C6H7O6.Na 198.106 g/mol
0.5 G_PER_L CHEBI:113451
sodium ascorbate

Preparation

  1. Adjust pH to 7.0 .
  2. Boil the medium for few minutes under nitrogen gas. Add sodium ascorbate (0.5 g) and continue to bubble with nitrogen gas.
  3. Adjust pH to 6.8.
  4. Fill immediately about 10 ml medium in 15 mL hungate tubes (with rubber septum).
  5. Autoclave at 121 °C for 15 min.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:10.922475Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 370
2026-03-13T06:19:04.428968Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001472
2026-03-17T06:29:47.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:16.450195+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "HELIOBACTERIUM CHLORUM MEDIUM" to "heliobacterium_chlorum_medium" for consistent matching
2026-06-05T07:41:08.367949+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:59.717146+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T10:55:41.454416+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.