natronobacteria_medium

CultureMech:001473 bacterial COMPLEX SOLID_AGAR
Original name: NATRONOBACTERIA MEDIUM

Overview

External ontology
mediadive.medium:371 (source link unavailable) — NATRONOBACTERIA MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 9.0, 'max': 9.5}
High-metal flag
True
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium371.pdf

Composition

flowchart LR
    CultureMech_001473["natronobacteria_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_001473 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_KCl["KCl"]
    CultureMech_001473 --> ing1_KCl
    CHEBI_32588["CHEBI:32588"]
    ing1_KCl -.-> CHEBI_32588
    ing2_NH4Cl["NH4Cl"]
    CultureMech_001473 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001473 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_CaSO4_x_2_H2O["CaSO4 x 2 H2O"]
    CultureMech_001473 --> ing4_CaSO4_x_2_H2O
    CHEBI_32583["CHEBI:32583"]
    ing4_CaSO4_x_2_H2O -.-> CHEBI_32583
    ing5_Agar["Agar"]
    CultureMech_001473 --> ing5_Agar
    CHEBI_2509["CHEBI:2509"]
    ing5_Agar -.-> CHEBI_2509
    ing6_NaCl["NaCl"]
    CultureMech_001473 --> ing6_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing6_NaCl -.-> CHEBI_26710
    ing7_Na2_glutamate["Na2-glutamate"]
    CultureMech_001473 --> ing7_Na2_glutamate
    CHEBI_64220["CHEBI:64220"]
    ing7_Na2_glutamate -.-> CHEBI_64220
    ing8_Yeast_extract["Yeast extract"]
    CultureMech_001473 --> ing8_Yeast_extract
    ing9_Casamino_acids["Casamino acids"]
    CultureMech_001473 --> ing9_Casamino_acids
    ing10_Na2CO3["Na2CO3"]
    CultureMech_001473 --> ing10_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing10_Na2CO3 -.-> CHEBI_29377
    ing11_HCl["HCl"]
    CultureMech_001473 --> ing11_HCl
    CHEBI_17883["CHEBI:17883"]
    ing11_HCl -.-> CHEBI_17883
    ing12_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_001473 --> ing12_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing12_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing13_ZnCl2["ZnCl2"]
    CultureMech_001473 --> ing13_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing13_ZnCl2 -.-> CHEBI_49976
    ing14_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001473 --> ing14_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing14_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing15_H3BO3["H3BO3"]
    CultureMech_001473 --> ing15_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing15_H3BO3 -.-> CHEBI_33118
    ing16_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001473 --> ing16_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing16_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing17_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001473 --> ing17_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing17_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing18_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001473 --> ing18_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing18_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing19_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001473 --> ing19_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing19_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (20)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
1 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 KCl
Cl.K 74.551 g/mol
1 G_PER_L CHEBI:32588
potassium chloride
3 NH4Cl
Cl.H4N 53.492 g/mol
1 G_PER_L CHEBI:31206
ammonium chloride
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.24 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 CaSO4 x 2 H2O
Ca.2H2O.O4S 172.171 g/mol
0.17 G_PER_L CHEBI:32583
calcium sulfate dihydrate
6 Agar 20 G_PER_L CHEBI:2509
agar
 (if necessary)
7 NaCl
Cl.Na 58.443 g/mol
200 G_PER_L CHEBI:26710
sodium chloride
8 Na2-glutamate
C5H8NO4.Na 169.112 g/mol
1 G_PER_L CHEBI:64220
monosodium glutamate
9 Yeast extract 5 G_PER_L
10 Casamino acids 5 G_PER_L
11 Na2CO3
CO3.2Na 105.988 g/mol
5 G_PER_L CHEBI:29377
sodium carbonate
12 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
13 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
14 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
15 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
16 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
17 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
18 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
19 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
20 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Add distilled water to give a final volume of 1000 ml.
  2. Adjust pH to 6.5 before autoclaving!
  3. Sterilize Na2CO3 separate from medium, add after cooling. Check final pH to be 9.0 - 9.5.
  4. If agar medium is prepared, heat and dissolve agar before adding sodium chloride.
  5. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:10.923963Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 371
2026-01-28T19:48:24.721875Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:04.437301Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001473
2026-03-17T06:30:17.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:16.458927+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "NATRONOBACTERIA MEDIUM" to "natronobacteria_medium" for consistent matching
2026-05-20T06:02:00.809796+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '9-9.5' -> {'min': 9.0, 'max': 9.5}
2026-06-05T07:41:39.370936+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 19 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:49.351923+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md.
2026-06-10T07:47:09.442026+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.