Overview
- External ontology
- mediadive.medium:372 (source link unavailable) — HALOBACTERIA MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 7.0, 'max': 7.2}
- High-metal flag
- True
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium372.pdf
Composition
flowchart LR
CultureMech_001474["halobacteria_medium"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_001474 --> ing0_Yeast_extract
ing1_Casamino_acids["Casamino acids"]
CultureMech_001474 --> ing1_Casamino_acids
ing2_Na_glutamate["Na glutamate"]
CultureMech_001474 --> ing2_Na_glutamate
CHEBI_64220["CHEBI:64220"]
ing2_Na_glutamate -.-> CHEBI_64220
ing3_KCl["KCl"]
CultureMech_001474 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_Na3_citrate["Na3-citrate"]
CultureMech_001474 --> ing4_Na3_citrate
CHEBI_53258["CHEBI:53258"]
ing4_Na3_citrate -.-> CHEBI_53258
ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001474 --> ing5_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
ing6_NaCl["NaCl"]
CultureMech_001474 --> ing6_NaCl
CHEBI_26710["CHEBI:26710"]
ing6_NaCl -.-> CHEBI_26710
ing7_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_001474 --> ing7_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing7_FeCl2_x_4_H2O -.-> CHEBI_86249
ing8_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_001474 --> ing8_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing8_MnCl2_x_4_H2O -.-> CHEBI_86368
ing9_Agar["Agar"]
CultureMech_001474 --> ing9_Agar
CHEBI_2509["CHEBI:2509"]
ing9_Agar -.-> CHEBI_2509
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 5 G_PER_L | ||
| 2 | Casamino acids | 5 G_PER_L | ||
| 3 |
Na glutamate
C5H8NO4.Na 169.112 g/mol
|
1 G_PER_L |
CHEBI:64220
monosodium glutamate
|
|
| 4 |
KCl
Cl.K 74.551 g/mol
|
2 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 5 |
Na3-citrate
C6H5O7.3Na 258.069 g/mol
|
3 G_PER_L |
CHEBI:53258
sodium citrate
|
|
| 6 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
20 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 7 |
NaCl
Cl.Na 58.443 g/mol
|
200 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 8 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
0.036 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 9 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.00036 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 10 | Agar | 20 G_PER_L |
CHEBI:2509
agar
|
Preparation
- Add distilled water to give a final volume of 1000 ml.
- Adjust pH to 7.0 - 7.2
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.927261Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 372 |
| 2026-01-28T19:48:16.027267Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:04.444739Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001474 |
| 2026-03-17T06:29:59.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:16.467183+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HALOBACTERIA MEDIUM" to "halobacteria_medium" for consistent matching |
| 2026-05-20T06:01:21.885490+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '7-7.2' -> {'min': 7.0, 'max': 7.2} |
| 2026-06-05T07:41:07.536742+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:39.636033+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-10-07T10:55:41.454416+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |