an1_medium

CultureMech:001477 bacterial COMPLEX LIQUID
Original name: AN1 MEDIUM

Overview

External ontology
mediadive.medium:376 (source link unavailable) — AN1 MEDIUM
Applications
  • Microbial cultivation
pH
7.3
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium376.pdf

Composition

flowchart LR
    CultureMech_001477["an1_medium"]
    ing0_Trypticase["Trypticase"]
    CultureMech_001477 --> ing0_Trypticase
    ing1_K2HPO4["K2HPO4"]
    CultureMech_001477 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_NaCl["NaCl"]
    CultureMech_001477 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_Na_thioglycolate["Na-thioglycolate"]
    CultureMech_001477 --> ing3_Na_thioglycolate
    CHEBI_86481["CHEBI:86481"]
    ing3_Na_thioglycolate -.-> CHEBI_86481
    ing4_Sulfur["Sulfur"]
    CultureMech_001477 --> ing4_Sulfur
    CHEBI_26833["CHEBI:26833"]
    ing4_Sulfur -.-> CHEBI_26833
    ing5_Resazurin["Resazurin"]
    CultureMech_001477 --> ing5_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing5_Resazurin -.-> CHEBI_8806

Ingredients (6)

# Ingredient Concentration Ontology Notes
1 Trypticase 10 G_PER_L MICRO:0000175 (source link unavailable)
Trypticase peptone
2 K2HPO4
HO4P.2K 174.174 g/mol
1.5 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
3 NaCl
Cl.Na 58.443 g/mol
2.5 G_PER_L CHEBI:26710
sodium chloride
4 Na-thioglycolate
C2H3O2S.Na 114.101 g/mol
1 G_PER_L CHEBI:86481
sodium thioglycolate
5 Sulfur
S 32.067 g/mol
8 G_PER_L CHEBI:26833
sulfur atom
6 Resazurin
C12H7NO4 229.191 g/mol
0.001 G_PER_L CHEBI:8806
Resazurin

Preparation

  1. Adjust pH to 7.3.
  2. Prepare the medium anaerobically under 100% nitrogen. Sterilize thioglycolate and sulfur separately. Sterilize the sulfur by steaming for 3 h on each of three successive days.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:10.942729Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 376
2026-01-28T19:48:37.849836Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:04.474984Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001477
2026-03-17T06:30:46.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:16.500686+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "AN1 MEDIUM" to "an1_medium" for consistent matching
2026-06-05T07:40:48.673819+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:53.089834+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-09-22T00:03:26.686152+00:00 repair_rejected_peptone_groundings.py CORRECTED_REJECTED_PEPTONE_GROUNDING CultureMech #484. /ingredients/0/term/id: Trypticase CHEBI:78018 -> MICRO:0000175 (replace_term). Reviewed identities: https://github.com/CultureBotAI/MediaIngredientMech/tree/24562493919391df4468a4285b7c997960cc8868. Publication resolver: MIM label index 9f09e4fb97fb0e6cbbd6f25baca40b36512adb88. MediaDive source IDs and concentrations preserved.
2026-10-07T10:55:41.454416+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.