Overview
- External ontology
- mediadive.medium:388 (source link unavailable) — DICTYOGLOMUS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium388.pdf
Composition
flowchart LR
CultureMech_001494["dictyoglomus_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_001494 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_Na2HPO4_x_12_H2O["Na2HPO4 x 12 H2O"]
CultureMech_001494 --> ing1_Na2HPO4_x_12_H2O
CHEBI_34683["CHEBI:34683"]
ing1_Na2HPO4_x_12_H2O -.-> CHEBI_34683
ing2_NH4Cl["NH4Cl"]
CultureMech_001494 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001494 --> ing3_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001494 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_Fe_NH4_2_SO4_2_x_6_H2O["Fe(NH4)2(SO4)2 x 6 H2O"]
CultureMech_001494 --> ing5_Fe_NH4_2_SO4_2_x_6_H2O
CHEBI_76181["CHEBI:76181"]
ing5_Fe_NH4_2_SO4_2_x_6_H2O -.-> CHEBI_76181
ing6_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_001494 --> ing6_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing6_CoCl2_x_6_H2O -.-> CHEBI_53503
ing7_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001494 --> ing7_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing7_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing8_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_001494 --> ing8_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing8_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing9_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_001494 --> ing9_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing9_MnCl2_x_4_H2O -.-> CHEBI_86368
ing10_ZnSO4["ZnSO4"]
CultureMech_001494 --> ing10_ZnSO4
CHEBI_35176["CHEBI:35176"]
ing10_ZnSO4 -.-> CHEBI_35176
ing11_Yeast_extract["Yeast extract"]
CultureMech_001494 --> ing11_Yeast_extract
ing12_Polypeptone["Polypeptone"]
CultureMech_001494 --> ing12_Polypeptone
ing13_Starch["Starch"]
CultureMech_001494 --> ing13_Starch
CHEBI_28017["CHEBI:28017"]
ing13_Starch -.-> CHEBI_28017
ing14_Sodium_resazurin["Sodium resazurin"]
CultureMech_001494 --> ing14_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing14_Sodium_resazurin -.-> CHEBI_8806
ing15_Na2CO3["Na2CO3"]
CultureMech_001494 --> ing15_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing15_Na2CO3 -.-> CHEBI_29377
ing16_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_001494 --> ing16_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing16_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing17_Biotin["Biotin"]
CultureMech_001494 --> ing17_Biotin
CHEBI_15956["CHEBI:15956"]
ing17_Biotin -.-> CHEBI_15956
ing18_Folic_acid["Folic acid"]
CultureMech_001494 --> ing18_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing18_Folic_acid -.-> CHEBI_27470
ing19_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_001494 --> ing19_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing19_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing20_Thiamine_HCl["Thiamine HCl"]
CultureMech_001494 --> ing20_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing20_Thiamine_HCl -.-> CHEBI_49105
ing21_Riboflavin["Riboflavin"]
CultureMech_001494 --> ing21_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing21_Riboflavin -.-> CHEBI_17015
ing22_Nicotinic_acid["Nicotinic acid"]
CultureMech_001494 --> ing22_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing22_Nicotinic_acid -.-> CHEBI_15940
ing23_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
CultureMech_001494 --> ing23_Calcium_D_____pantothenate
CHEBI_31345["CHEBI:31345"]
ing23_Calcium_D_____pantothenate -.-> CHEBI_31345
ing24_Vitamin_B12["Vitamin B12"]
CultureMech_001494 --> ing24_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing24_Vitamin_B12 -.-> CHEBI_176843
ing25_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_001494 --> ing25_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing25_p_Aminobenzoic_acid -.-> CHEBI_30753
ing26__DL__alpha_Lipoic_acid["(DL)-alpha-Lipoic acid"]
CultureMech_001494 --> ing26__DL__alpha_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing26__DL__alpha_Lipoic_acid -.-> CHEBI_16494
Ingredients (27)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.48075 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
Na2HPO4 x 12 H2O
HO4P.2Na 141.958 g/mol
|
4.1461 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.493583 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.375123 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.05923 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 6 |
Fe(NH4)2(SO4)2 x 6 H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
|
0.0394867 G_PER_L |
CHEBI:76181
ferrous ammonium sulfate hexahydrate
|
|
| 7 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.00286278 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 8 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.0023692 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 9 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.000167818 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 10 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.00197433 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 11 |
ZnSO4
O4S.Zn 161.453 g/mol
|
0.00276407 G_PER_L |
CHEBI:35176
zinc sulfate
|
|
| 12 | Yeast extract | 1.97433 G_PER_L | ||
| 13 | Polypeptone | 1.97433 G_PER_L | ||
| 14 | Starch | 4.93583 G_PER_L |
CHEBI:28017
starch
|
|
| 15 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.000493583 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 16 |
Na2CO3
CO3.2Na 105.988 g/mol
|
0.987167 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 17 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.987167 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 18 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 19 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
|
| 20 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.1 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 21 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.05 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 22 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 23 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.05 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 24 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.05 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 25 | Vitamin B12 | 0.001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 26 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 27 |
(DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.05 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Dissolve ingredients (except carbonate, cysteine and vitamins), bring medium to the boil, then cool to room temperature under 100% N2 gas atmosphere. Add and dissolve carbonate and cysteine, adjust pH to 7.2, then dispense under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add vitamins from an anoxic stock solution prepared under 100% N2 gas and sterilized by filtration. Adjust pH of complete medium to 7.2, if necessary.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.001747Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 388 |
| 2026-01-28T19:48:20.567617Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-06T05:10:28.743651Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T06:19:04.668082Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001494 |
| 2026-03-17T06:30:09.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:16.717101+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "DICTYOGLOMUS MEDIUM" to "dictyoglomus_medium" for consistent matching |
| 2026-06-05T07:40:58.693552+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 23 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:56.372655+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |