annwoodia_medium

CultureMech:001495 bacterial DEFINED SOLID_AGAR
Original name: ANNWOODIA MEDIUM

Overview

External ontology
mediadive.medium:389 (source link unavailable) — ANNWOODIA MEDIUM
Applications
  • Microbial cultivation
pH
7.6
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium389.pdf

Composition

flowchart LR
    CultureMech_001495["annwoodia_medium"]
    ing0_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
    CultureMech_001495 --> ing0_Na2HPO4_x_2_H2O
    CHEBI_91258["CHEBI:91258"]
    ing0_Na2HPO4_x_2_H2O -.-> CHEBI_91258
    ing1_KH2PO4["KH2PO4"]
    CultureMech_001495 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_NH4Cl["NH4Cl"]
    CultureMech_001495 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001495 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_Agar["Agar"]
    CultureMech_001495 --> ing4_Agar
    CHEBI_2509["CHEBI:2509"]
    ing4_Agar -.-> CHEBI_2509
    ing5_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
    CultureMech_001495 --> ing5_Na2S2O3_x_5_H2O
    CHEBI_32150["CHEBI:32150"]
    ing5_Na2S2O3_x_5_H2O -.-> CHEBI_32150
    ing6_Na2_EDTA["Na2-EDTA"]
    CultureMech_001495 --> ing6_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing6_Na2_EDTA -.-> CHEBI_64734
    ing7_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001495 --> ing7_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing7_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing8_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001495 --> ing8_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing8_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing9__NH4_6Mo7O24_x_4_H2O["(NH4)6Mo7O24 x 4 H2O"]
    CultureMech_001495 --> ing9__NH4_6Mo7O24_x_4_H2O
    CHEBI_91249["CHEBI:91249"]
    ing9__NH4_6Mo7O24_x_4_H2O -.-> CHEBI_91249
    ing10_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001495 --> ing10_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing10_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing11_NaOH["NaOH"]
    CultureMech_001495 --> ing11_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing11_NaOH -.-> CHEBI_32145

Ingredients (12)

# Ingredient Concentration Ontology Notes
1 Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
79 G_PER_L CHEBI:91258
disodium hydrogenphosphate dihydrate
2 KH2PO4
H2O4P.K 136.084 g/mol
15 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 NH4Cl
Cl.H4N 53.492 g/mol
0.45977 G_PER_L CHEBI:31206
ammonium chloride
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.114943 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 Agar 17.2414 G_PER_L CHEBI:2509
agar
 (for solid medium)
6 Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
250 G_PER_L CHEBI:32150
sodium thiosulfate pentahydrate
7 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
50 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
8 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
7.34 G_PER_L CHEBI:86158
calcium chloride dihydrate
9 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.5 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
10 (NH4)6Mo7O24 x 4 H2O
2H4N.MoO4 196.014 g/mol
0.5 G_PER_L CHEBI:91249
ammonium molybdate
11 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.2 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
12 NaOH
HO.Na 39.997 g/mol
11 G_PER_L CHEBI:32145
sodium hydroxide

Preparation

  1. Autoclave solutions A, B and C and filter sterilize solution D. To complete the medium combine sterile solutions when cool.
  2. Adjust to pH 7.6.
  3. Dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds. Adjust pH of final solution to 6.0 with NaOH.

Solutions

Trace element solution

Dose: 10 ML_PER_L

Stock composition
IngredientConcentrationNotes
ZnSO4 x 7 H2O 11 G_PER_L
MnCl2 x 4 H2O 2.5 G_PER_L
FeSO4 x 7 H2O 5 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:11.005432Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 389
2026-03-13T06:19:04.677382Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001495
2026-03-17T06:30:07.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:16.727832+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ANNWOODIA MEDIUM" to "annwoodia_medium" for consistent matching
2026-06-05T07:40:49.217822+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:53.355525+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-07T07:05:21.622719+00:00 apply_cocktail_nesting.py NESTED_FLATTENED_COCKTAIL Moved 3 stock-strength component(s) out of ingredients into 1 solution(s): 3 -> 'Trace element solution' @ 10 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150).