Overview
- External ontology
- mediadive.medium:38 (source link unavailable) — ACETOANAEROBIUM MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium38.pdf
Composition
flowchart LR
CultureMech_001496["acetoanaerobium_medium"]
ing0_Tryptone["Tryptone"]
CultureMech_001496 --> ing0_Tryptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_001496 --> ing1_Yeast_extract
ing2_K2HPO4["K2HPO4"]
CultureMech_001496 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_KH2PO4["KH2PO4"]
CultureMech_001496 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_Sodium_resazurin["Sodium resazurin"]
CultureMech_001496 --> ing4_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing4_Sodium_resazurin -.-> CHEBI_8806
ing5_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_001496 --> ing5_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing5_Na2S_x_9_H2O -.-> CHEBI_76209
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Tryptone | 20 G_PER_L | ||
| 2 | Yeast extract | 10 G_PER_L | ||
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1.04 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.68 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 5 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.0005 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 6 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.15 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Preparation
- Dissolve ingredients (except sulfide), adjust pH to 7.0, bring medium to the boil, then cool to room temperature under 100% N2 gas. Dispense under same gas atmosphere in culture vessels and autoclave. After sterilization add sulfide from a sterile anoxic stock solution prepared under 100% N2 gas.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.024554Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 38 |
| 2026-03-13T06:19:04.682364Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001496 |
| 2026-03-17T06:29:58.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:16.733524+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ACETOANAEROBIUM MEDIUM" to "acetoanaerobium_medium" for consistent matching |
| 2026-06-05T07:40:46.703495+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:52.379436+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-07T10:55:41.454416+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |