Overview
- External ontology
- mediadive.medium:390 (source link unavailable) — PYROBACULUM MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium390.pdf
Composition
flowchart LR
CultureMech_001497["pyrobaculum_medium"]
ing0__NH4_2SO4["(NH4)2SO4"]
CultureMech_001497 --> ing0__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing0__NH4_2SO4 -.-> CHEBI_62946
ing1_KH2PO4["KH2PO4"]
CultureMech_001497 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001497 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001497 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_001497 --> ing4_FeCl3_x_6_H2O
CHEBI_86254["CHEBI:86254"]
ing4_FeCl3_x_6_H2O -.-> CHEBI_86254
ing5_Sodium_resazurin["Sodium resazurin"]
CultureMech_001497 --> ing5_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing5_Sodium_resazurin -.-> CHEBI_8806
ing6_Trypticase_peptone["Trypticase peptone"]
CultureMech_001497 --> ing6_Trypticase_peptone
ing7_Yeast_extract["Yeast extract"]
CultureMech_001497 --> ing7_Yeast_extract
ing8_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
CultureMech_001497 --> ing8_Na2S2O3_x_5_H2O
CHEBI_32150["CHEBI:32150"]
ing8_Na2S2O3_x_5_H2O -.-> CHEBI_32150
ing9_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_001497 --> ing9_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing9_Na2S_x_9_H2O -.-> CHEBI_76209
ing10_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_001497 --> ing10_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing10_MnCl2_x_4_H2O -.-> CHEBI_86368
ing11_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
CultureMech_001497 --> ing11_Na2B4O7_x_10_H2O
CHEBI_131366["CHEBI:131366"]
ing11_Na2B4O7_x_10_H2O -.-> CHEBI_131366
ing12_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001497 --> ing12_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing12_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing13_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_001497 --> ing13_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing13_CuCl2_x_2_H2O -.-> CHEBI_86318
ing14_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001497 --> ing14_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing14_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing15_VOSO4_x_2_H2O["VOSO4 x 2 H2O"]
CultureMech_001497 --> ing15_VOSO4_x_2_H2O
CHEBI_87009["CHEBI:87009"]
ing15_VOSO4_x_2_H2O -.-> CHEBI_87009
ing16_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_001497 --> ing16_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing16_CoSO4_x_7_H2O -.-> CHEBI_91244
Ingredients (17)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1.28713 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.277228 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.247525 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.0693069 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
0.019802 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
|
| 6 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.00049505 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 7 | Trypticase peptone | 0.49505 G_PER_L | ||
| 8 | Yeast extract | 0.19802 G_PER_L | ||
| 9 |
Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
|
1.9802 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
|
| 10 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.49505 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 11 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.18 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 12 |
Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
|
0.45 G_PER_L |
CHEBI:131366
disodium tetraborate decahydrate
|
|
| 13 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.022 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 14 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.005 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 15 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.003 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 16 |
VOSO4 x 2 H2O
2H2O.O4S.OV 199.036 g/mol
|
0.003 G_PER_L |
CHEBI:87009
vanadyl sulfate dihydrate
|
|
| 17 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.001 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
Preparation
- Dissolve ingredients (except Trypticase peptone, yeast extract, sodium thiosulfate and sulfide), adjust pH to 6.0 and sparge medium with 100% N2 gas for at least 30 min to remove dissolved oxygen. After autoclaving add peptone, yeast extract, thiosulfate and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Stock solutions of substrates can be autoclaved with the exception of sodium thiosulfate which should be sterilized by filtration. Prior to inoculation adjust pH of final medium to 6.0.
- Adjust pH of final solution to 2 with 1 N HCl.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.007874Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 390 |
| 2026-03-13T06:19:04.688992Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001497 |
| 2026-03-17T06:29:40.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:16.740623+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PYROBACULUM MEDIUM" to "pyrobaculum_medium" for consistent matching |
| 2026-06-05T07:41:43.248931+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:10.647518+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-13T00:00:00-07:00 | repair_komodo_390_pyrobaculum_score10.py | RESOLVED_KOMODO_390_PYROBACULUM_STRAIN_VARIANTS | Pointed the DSMZ parent at the actual archaeal KOMODO Medium 390 file. |