Overview
- External ontology
- mediadive.medium:392 (source link unavailable) — BAF Medium
- Applications
-
- Microbial cultivation
- pH range
- {'min': 5.8, 'max': 6.3}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium392.pdf
Composition
flowchart LR
CultureMech_001499["baf_medium"]
ing0_Glucose["Glucose"]
CultureMech_001499 --> ing0_Glucose
CHEBI_17234["CHEBI:17234"]
ing0_Glucose -.-> CHEBI_17234
ing1_Peptone["Peptone"]
CultureMech_001499 --> ing1_Peptone
ing2_Yeast_extract["Yeast extract"]
CultureMech_001499 --> ing2_Yeast_extract
ing3_KH2PO4["KH2PO4"]
CultureMech_001499 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001499 --> ing4_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
ing5_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_001499 --> ing5_FeCl3_x_6_H2O
CHEBI_86254["CHEBI:86254"]
ing5_FeCl3_x_6_H2O -.-> CHEBI_86254
ing6_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001499 --> ing6_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing6_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing7_MnSO4["MnSO4"]
CultureMech_001499 --> ing7_MnSO4
CHEBI_86360["CHEBI:86360"]
ing7_MnSO4 -.-> CHEBI_86360
ing8_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001499 --> ing8_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing8_CaCl2_x_2_H2O -.-> CHEBI_86158
ing9_Thiamine_HCl["Thiamine HCl"]
CultureMech_001499 --> ing9_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing9_Thiamine_HCl -.-> CHEBI_49105
ing10_Biotin["Biotin"]
CultureMech_001499 --> ing10_Biotin
CHEBI_15956["CHEBI:15956"]
ing10_Biotin -.-> CHEBI_15956
ing11_Folic_acid["Folic acid"]
CultureMech_001499 --> ing11_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing11_Folic_acid -.-> CHEBI_27470
ing12_Inositol["Inositol"]
CultureMech_001499 --> ing12_Inositol
CHEBI_24848["CHEBI:24848"]
ing12_Inositol -.-> CHEBI_24848
ing13_Agar["Agar"]
CultureMech_001499 --> ing13_Agar
CHEBI_2509["CHEBI:2509"]
ing13_Agar -.-> CHEBI_2509
Ingredients (14)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Glucose
C6H12O6 180.156 g/mol
|
30 G_PER_L |
CHEBI:17234
glucose
|
|
| 2 | Peptone | 2 G_PER_L | ||
| 3 | Yeast extract | 0.2 G_PER_L | ||
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 5 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 6 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
0.01 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
|
| 7 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.001 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 8 |
MnSO4
Mn.O4S 151.001 g/mol
|
0.005 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 9 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 10 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
5e-05 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 11 |
Biotin
C10H16N2O3S 244.316 g/mol
|
1e-06 G_PER_L |
CHEBI:15956
biotin
|
|
| 12 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.0001 G_PER_L |
CHEBI:27470
folic acid
|
|
| 13 |
Inositol
C6H12O6 180.156 g/mol
|
5e-05 G_PER_L |
CHEBI:24848
inositol
|
|
| 14 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Preparation
- pH 5.8 - 6.3. Sterilize at 121°C for 15 min.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.014974Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 392 |
| 2026-03-13T06:19:04.710713Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001499 |
| 2026-03-17T06:30:45.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:16.764965+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "BAF Medium" to "baf_medium" for consistent matching |
| 2026-05-20T06:01:09.577982+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '5.8-6.3' -> {'min': 5.8, 'max': 6.3} |
| 2026-06-05T07:40:50.475447+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:53.784973+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-07T10:55:41.454416+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |