Overview
- External ontology
- mediadive.medium:394 (source link unavailable) — CHI 1776 MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium394.pdf
Composition
flowchart LR
CultureMech_001501["chi_1776_medium"]
ing0_Tryptone["Tryptone"]
CultureMech_001501 --> ing0_Tryptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_001501 --> ing1_Yeast_extract
ing2_Tris_HCl["Tris-HCl"]
CultureMech_001501 --> ing2_Tris_HCl
ing3_MgCl2["MgCl2"]
CultureMech_001501 --> ing3_MgCl2
CHEBI_6636["CHEBI:6636"]
ing3_MgCl2 -.-> CHEBI_6636
ing4_Diaminopimelic_acid["Diaminopimelic acid"]
CultureMech_001501 --> ing4_Diaminopimelic_acid
CHEBI_23673["CHEBI:23673"]
ing4_Diaminopimelic_acid -.-> CHEBI_23673
ing5_Thymidine["Thymidine"]
CultureMech_001501 --> ing5_Thymidine
CHEBI_17748["CHEBI:17748"]
ing5_Thymidine -.-> CHEBI_17748
ing6_Glucose["Glucose"]
CultureMech_001501 --> ing6_Glucose
CHEBI_17234["CHEBI:17234"]
ing6_Glucose -.-> CHEBI_17234
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Tryptone | 23.3645 G_PER_L | ||
| 2 | Yeast extract | 7.00935 G_PER_L | ||
| 3 | Tris-HCl | 20 G_PER_L | ||
| 4 |
MgCl2
2Cl.Mg 95.211 g/mol
|
0.47605 G_PER_L |
CHEBI:6636
magnesium dichloride
|
|
| 5 |
Diaminopimelic acid
C7H14N2O4 190.199 g/mol
|
0.1 G_PER_L |
CHEBI:23673
2,6-diaminopimelic acid
|
|
| 6 |
Thymidine
C10H14N2O5 242.231 g/mol
|
0.04 G_PER_L |
CHEBI:17748
thymidine
|
|
| 7 |
Glucose
C6H12O6 180.156 g/mol
|
5 G_PER_L |
CHEBI:17234
glucose
|
Preparation
- Autoclave, cool, and then add:
- Magnesium chloride should be sterilized separately by autoclaving, and the other ingredients should be sterilized separately by filtration.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 2 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable). |
| 2026-01-26T17:53:11.018174Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 394 |
| 2026-02-06T05:10:26.653297Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T06:19:04.718120Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001501 |
| 2026-03-17T06:30:05.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:16.773569+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CHI 1776 MEDIUM" to "chi_1776_medium" for consistent matching |
| 2026-06-05T07:40:53.251890+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-10-07T10:55:41.454416+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |