Overview
- External ontology
- mediadive.medium:396 (source link unavailable) — METHANOSALSUM ZHILINAE MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 9.2, 'max': 9.4}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium396.pdf
Composition
flowchart LR
CultureMech_001505["methanosalsum_zhilinae_medium"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_001505 --> ing0_Yeast_extract
ing1_Trypticase_peptone["Trypticase peptone"]
CultureMech_001505 --> ing1_Trypticase_peptone
ing2_NaCl["NaCl"]
CultureMech_001505 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_NH4Cl["NH4Cl"]
CultureMech_001505 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001505 --> ing4_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001505 --> ing5_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
ing6_KCl["KCl"]
CultureMech_001505 --> ing6_KCl
CHEBI_32588["CHEBI:32588"]
ing6_KCl -.-> CHEBI_32588
ing7_K2HPO4["K2HPO4"]
CultureMech_001505 --> ing7_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing7_K2HPO4 -.-> CHEBI_131527
ing8_Sodium_resazurin["Sodium resazurin"]
CultureMech_001505 --> ing8_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing8_Sodium_resazurin -.-> CHEBI_8806
ing9_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_001505 --> ing9_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing9_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing10_NaHCO3["NaHCO3"]
CultureMech_001505 --> ing10_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing10_NaHCO3 -.-> CHEBI_32139
ing11_Na2CO3["Na2CO3"]
CultureMech_001505 --> ing11_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing11_Na2CO3 -.-> CHEBI_29377
ing12_Trimethylamine_HCl["Trimethylamine-HCl"]
CultureMech_001505 --> ing12_Trimethylamine_HCl
CHEBI_64700["CHEBI:64700"]
ing12_Trimethylamine_HCl -.-> CHEBI_64700
ing13_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_001505 --> ing13_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing13_Na2S_x_9_H2O -.-> CHEBI_76209
ing14_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_001505 --> ing14_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing14_Nitrilotriacetic_acid -.-> CHEBI_44557
ing15_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_001505 --> ing15_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing15_MnSO4_x_H2O -.-> CHEBI_86364
ing16_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001505 --> ing16_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing16_FeSO4_x_7_H2O -.-> CHEBI_75836
ing17_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_001505 --> ing17_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing17_CoSO4_x_7_H2O -.-> CHEBI_91244
ing18_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001505 --> ing18_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing18_CaCl2_x_2_H2O -.-> CHEBI_86158
ing19_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001505 --> ing19_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing19_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing20_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_001505 --> ing20_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing20_CuSO4_x_5_H2O -.-> CHEBI_31440
ing21_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_001505 --> ing21_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing21_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing22_H3BO3["H3BO3"]
CultureMech_001505 --> ing22_H3BO3
CHEBI_33118["CHEBI:33118"]
ing22_H3BO3 -.-> CHEBI_33118
ing23_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001505 --> ing23_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing23_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing24_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001505 --> ing24_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing24_NiCl2_x_6_H2O -.-> CHEBI_34887
ing25_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_001505 --> ing25_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing25_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing26_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_001505 --> ing26_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing26_Na2WO4_x_2_H2O -.-> CHEBI_63939
Ingredients (27)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 2 G_PER_L | ||
| 2 | Trypticase peptone | 2 G_PER_L | ||
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
41.0 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 40.0, 1.0] |
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 5 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
3.5 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 6 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3.0 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
[Collapsed 2 identical duplicates: 3.0, 3.0] |
| 7 |
KCl
Cl.K 74.551 g/mol
|
1 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 8 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.4 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 9 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.0005 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 10 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 11 |
NaHCO3
CHO3.Na 84.006 g/mol
|
3 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 12 |
Na2CO3
CO3.2Na 105.988 g/mol
|
2 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 13 |
Trimethylamine-HCl
C3H9N.HCl 95.573 g/mol
|
2 G_PER_L |
CHEBI:64700
trimethylamine hydrochloride
|
|
| 14 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.25 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 15 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 16 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 17 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 18 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.18 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 19 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 20 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.18 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 21 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 22 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.02 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 23 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 24 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 25 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.03 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 26 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.0003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 27 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.0004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Preparation
- Dissolve ingredients except cysteine, bicarbonate, carbonate, trimethylamine and sulfide, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add cysteine and dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add trimethylamine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas, whereas bicarbonate and carbonate are added from sterile anoxic stock solutions prepared under 80% N2 and 20% CO2 gas atmosphere. Adjust pH of complete medium to 9.2 - 9.4.
- First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.031996Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 396 |
| 2026-01-28T19:48:33.113504Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T03:09:26.330171Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:04.768303Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001505 |
| 2026-03-17T06:30:35.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:16.828776+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "METHANOSALSUM ZHILINAE MEDIUM" to "methanosalsum_zhilinae_medium" for consistent matching |
| 2026-05-20T06:01:56.235433+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '9.2-9.4' -> {'min': 9.2, 'max': 9.4} |
| 2026-06-05T07:41:32.325394+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 24 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:07.272793+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-02T07:37:30.739128+00:00 | repair_merged_duplicates.py | REPAIRED_SUMMED_DUPLICATE_MERGE | Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched. |