thermoplasma_volcanium_medium

CultureMech:001507 archaea COMPLEX LIQUID
Original name: THERMOPLASMA VOLCANIUM MEDIUM

Overview

External ontology
mediadive.medium:398 (source link unavailable) — THERMOPLASMA VOLCANIUM MEDIUM
Applications
  • Microbial cultivation
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium398.pdf

Composition

flowchart LR
    CultureMech_001507["thermoplasma_volcanium_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_001507 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001507 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001507 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3__NH4_2SO4["(NH4)2SO4"]
    CultureMech_001507 --> ing3__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing3__NH4_2SO4 -.-> CHEBI_62946
    ing4_Yeast_extract["Yeast extract"]
    CultureMech_001507 --> ing4_Yeast_extract
    ing5_Glucose["Glucose"]
    CultureMech_001507 --> ing5_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing5_Glucose -.-> CHEBI_17234
    ing6_Sulfur["Sulfur"]
    CultureMech_001507 --> ing6_Sulfur
    CHEBI_26833["CHEBI:26833"]
    ing6_Sulfur -.-> CHEBI_26833

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
3 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
1 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
3 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.25 G_PER_L CHEBI:86158
calcium chloride dihydrate
4 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.2 G_PER_L CHEBI:62946
ammonium sulfate
5 Yeast extract 1 G_PER_L
6 Glucose
C6H12O6 180.156 g/mol
5 G_PER_L CHEBI:17234
glucose
7 Sulfur
S 32.067 g/mol
4 G_PER_L CHEBI:26833
sulfur atom
 (for anaerobic media only)

Preparation

  1. Adjust pH of the mineral base to about 2 with sulfuric acid and autoclave. Add glucose and yeast extract from filter-sterilized stock solutions.
  2. Check pH to be 2 to 3.
  3. For anaerobic growth, the acidified mineral base containing sulfur is filled into serum bottles (20 ml per 100 ml bottle) under N2 + CO2 (80 + 20) gas mixture. Then, the bottles are sterilized by tyndallisation. Yeast extract and glucose are added from separately sterilized stock solutions.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:11.040610Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 398
2026-03-13T06:19:04.790397Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001507
2026-03-17T06:30:36.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:16.853954+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "THERMOPLASMA VOLCANIUM MEDIUM" to "thermoplasma_volcanium_medium" for consistent matching
2026-06-05T07:41:50.421598+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:13.117940+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-09-13T00:00:00-07:00 repair_komodo_398_thermoplasma_score10.py RESOLVED_KOMODO_398_THERMOPLASMA_TOPOLOGY Moved the family root from strain-specific KOMODO Medium 398.1 to the DSMZ/MediaDive THERMOPLASMA VOLCANIUM MEDIUM record.
2026-10-07T10:55:41.454416+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.