archaeoglobus_medium (CultureMech:001508)

Overview

External ontology

mediadive.medium:399 (source link unavailable) — ARCHAEOGLOBUS MEDIUM

Applications

Microbial cultivation

pH

6.9

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium399.pdf

Composition

flowchart LR
    CultureMech_001508["archaeoglobus_medium"]
    ing0_KCl["KCl"]
    CultureMech_001508 --> ing0_KCl
    CHEBI_32588["CHEBI:32588"]
    ing0_KCl -.-> CHEBI_32588
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001508 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001508 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_NH4Cl["NH4Cl"]
    CultureMech_001508 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001508 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_K2HPO4["K2HPO4"]
    CultureMech_001508 --> ing5_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing5_K2HPO4 -.-> CHEBI_131527
    ing6_NaCl["NaCl"]
    CultureMech_001508 --> ing6_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing6_NaCl -.-> CHEBI_26710
    ing7_Yeast_extract["Yeast extract"]
    CultureMech_001508 --> ing7_Yeast_extract
    ing8_Na_L_lactate["Na-L-lactate"]
    CultureMech_001508 --> ing8_Na_L_lactate
    CHEBI_232798["CHEBI:232798"]
    ing8_Na_L_lactate -.-> CHEBI_232798
    ing9_Fe_NH4_2_SO4_2_x_7_H2O["Fe(NH4)2(SO4)2 x 7 H2O"]
    CultureMech_001508 --> ing9_Fe_NH4_2_SO4_2_x_7_H2O
    CHEBI_131378["CHEBI:131378"]
    ing9_Fe_NH4_2_SO4_2_x_7_H2O -.-> CHEBI_131378
    ing10_Sodium_resazurin["Sodium resazurin"]
    CultureMech_001508 --> ing10_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing10_Sodium_resazurin -.-> CHEBI_8806
    ing11_NaHCO3["NaHCO3"]
    CultureMech_001508 --> ing11_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing11_NaHCO3 -.-> CHEBI_32139
    ing12_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_001508 --> ing12_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing12_Na2S_x_9_H2O -.-> CHEBI_76209
    ing13_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_001508 --> ing13_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing13_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing14_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_001508 --> ing14_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing14_MnSO4_x_H2O -.-> CHEBI_86364
    ing15_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001508 --> ing15_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing15_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing16_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_001508 --> ing16_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing16_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing17_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001508 --> ing17_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing17_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing18_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001508 --> ing18_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing18_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing19_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_001508 --> ing19_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing19_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing20_H3BO3["H3BO3"]
    CultureMech_001508 --> ing20_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing20_H3BO3 -.-> CHEBI_33118
    ing21_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001508 --> ing21_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing21_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing22_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001508 --> ing22_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing22_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing23_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_001508 --> ing23_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing23_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing24_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_001508 --> ing24_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing24_Na2WO4_x_2_H2O -.-> CHEBI_63939

Ingredients (25)

#	Ingredient	Concentration	Ontology	Notes
1	KCl Cl.K 74.551 g/mol	0.335968 G_PER_L	CHEBI:32588 potassium chloride
2	MgCl2 x 6 H2O 2Cl.6H2O.Mg 203.301 g/mol	3.95257 G_PER_L	CHEBI:86345 magnesium dichloride hexahydrate
3	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol	6.40909 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate	[Merged 2 duplicates: 3.40909, 3.0]
4	NH4Cl Cl.H4N 53.492 g/mol	0.247036 G_PER_L	CHEBI:31206 ammonium chloride
5	CaCl2 x 2 H2O Ca.2Cl.2H2O 147.014 g/mol	0.23834 G_PER_L	CHEBI:86158 calcium chloride dihydrate	[Merged 2 duplicates: 0.13834, 0.1]
6	K2HPO4 HO4P.2K 174.174 g/mol	0.13834 G_PER_L	CHEBI:131527 dipotassium hydrogen phosphate
7	NaCl Cl.Na 58.443 g/mol	18.7866 G_PER_L	CHEBI:26710 sodium chloride	[Merged 2 duplicates: 17.7866, 1.0]
8	Yeast extract	0.494071 G_PER_L
9	Na-L-lactate C3H5O3.Na 112.06 g/mol	1.48221 G_PER_L	CHEBI:232798 sodium L-lactate
10	Fe(NH4)2(SO4)2 x 7 H2O Fe.7H2O.2H4N.2O4S 410.154 g/mol	0.00197628 G_PER_L	CHEBI:131378 ferrous ammonium sulfate heptahydrate
11	Sodium resazurin C12H7NO4 229.191 g/mol	0.000494071 G_PER_L	CHEBI:8806 Resazurin
12	NaHCO3 CHO3.Na 84.006 g/mol	2.96443 G_PER_L	CHEBI:32139 sodium hydrogencarbonate
13	Na2S x 9 H2O 9H2O.2Na.S 240.182 g/mol	0.494071 G_PER_L	CHEBI:76209 sodium sulfide nonahydrate
14	Nitrilotriacetic acid C6H9NO6 191.139 g/mol	1.5 G_PER_L	CHEBI:44557 nitrilotriacetic acid
15	MnSO4 x H2O H2O.Mn.O4S 169.016 g/mol	0.5 G_PER_L	CHEBI:86364 manganese(II) sulfate monohydrate
16	FeSO4 x 7 H2O Fe.7H2O.O4S 278.013 g/mol	0.1 G_PER_L	CHEBI:75836 iron(2+) sulfate heptahydrate
17	CoSO4 x 7 H2O Co.7H2O.O4S 281.101 g/mol	0.18 G_PER_L	CHEBI:91244 cobalt(2+) sulfate heptahydrate
18	ZnSO4 x 7 H2O 7H2O.O4S.Zn 287.558 g/mol	0.18 G_PER_L	CHEBI:32312 zinc sulfate heptahydrate
19	CuSO4 x 5 H2O Cu.5H2O.O4S 249.684 g/mol	0.01 G_PER_L	CHEBI:31440 copper(II) sulfate pentahydrate
20	AlK(SO4)2 x 12 H2O Al.12H2O.K.2O4S 474.386 g/mol	0.02 G_PER_L	CHEBI:86465 potassium aluminium sulfate dodecahydrate
21	H3BO3 H3BO3 61.833 g/mol	0.01 G_PER_L	CHEBI:33118 boric acid
22	Na2MoO4 x 2 H2O 2H2O.MoO4.2Na 241.946 g/mol	0.01 G_PER_L	CHEBI:75213 sodium molybdate dihydrate
23	NiCl2 x 6 H2O Cl2Ni 129.599 g/mol	0.03 G_PER_L	CHEBI:34887 nickel dichloride
24	Na2SeO3 x 5 H2O 5H2O.2Na.O3Se 263.012 g/mol	0.0003 G_PER_L	CHEBI:131361 disodium selenite pentahydrate
25	Na2WO4 x 2 H2O 2H2O.2Na.O4W 329.846 g/mol	0.0004 G_PER_L	CHEBI:63939 sodium tungstate dihydrate

Preparation

Dissolve ingredients except bicarbonate and sulfide, then sparge medium with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add bicarbonate and adjust pH to 6.9, then dispense medium under 80% H2 and 20% CO2 gas atmosphere into serum vials (e.g., 20 ml per 100 ml bottle), seal and autoclave. Before use reduce the medium with sodium sulfide from a sterile anoxic stock solution prepared under 100% N2 gas.
After inoculation, add 2 bar overpressure of sterile 80% H2 and 20% CO2 gas mixture.
First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-26T17:53:11.042377Z	mediadive-import	Imported from MediaDive	Source: DSMZ, ID: 399
2026-01-28T19:48:17.503802Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T03:08:57.822496Z	data-quality-cleanup-v1.0	Data quality fixes	Merged 3 duplicate ingredient(s)
2026-03-13T06:19:04.800326Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:001508
2026-03-17T06:30:02.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:16.864889+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "ARCHAEOGLOBUS MEDIUM" to "archaeoglobus_medium" for consistent matching
2026-06-05T07:40:49.498438+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 23 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:53.451154+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.