azotobacter_medium

CultureMech:001510 bacterial DEFINED SOLID_AGAR
Original name: AZOTOBACTER MEDIUM

Overview

External ontology
mediadive.medium:3 (source link unavailable) — AZOTOBACTER MEDIUM
Applications
  • Microbial cultivation
pH
7.3
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium3.pdf

Composition

flowchart LR
    CultureMech_001510["azotobacter_medium"]
    ing0_Glucose["Glucose"]
    CultureMech_001510 --> ing0_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing0_Glucose -.-> CHEBI_17234
    ing1_Mannitol["Mannitol"]
    CultureMech_001510 --> ing1_Mannitol
    CHEBI_29864["CHEBI:29864"]
    ing1_Mannitol -.-> CHEBI_29864
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001510 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001510 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001510 --> ing4_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing4_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing5_K2HPO4["K2HPO4"]
    CultureMech_001510 --> ing5_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing5_K2HPO4 -.-> CHEBI_131527
    ing6_KH2PO4["KH2PO4"]
    CultureMech_001510 --> ing6_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing6_KH2PO4 -.-> CHEBI_63036
    ing7_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001510 --> ing7_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing7_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing8_CaCO3["CaCO3"]
    CultureMech_001510 --> ing8_CaCO3
    CHEBI_3311["CHEBI:3311"]
    ing8_CaCO3 -.-> CHEBI_3311
    ing9_Agar["Agar"]
    CultureMech_001510 --> ing9_Agar
    CHEBI_2509["CHEBI:2509"]
    ing9_Agar -.-> CHEBI_2509

Ingredients (10)

# Ingredient Concentration Ontology Notes
1 Glucose
C6H12O6 180.156 g/mol
5 G_PER_L CHEBI:17234
glucose
2 Mannitol
C6H14O6 182.172 g/mol
5 G_PER_L CHEBI:29864
mannitol
3 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.1 G_PER_L CHEBI:86158
calcium chloride dihydrate
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.1 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.005 G_PER_L CHEBI:75213
sodium molybdate dihydrate
6 K2HPO4
HO4P.2K 174.174 g/mol
0.9 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
7 KH2PO4
H2O4P.K 136.084 g/mol
0.1 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
8 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.01 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
9 CaCO3
CO3.Ca 100.086 g/mol
5 G_PER_L CHEBI:3311
calcium carbonate
10 Agar 15 G_PER_L CHEBI:2509
agar

Preparation

  1. Adjust pH to 7.3.
  2. Sterilize glucose and mannitol separately (in 50 ml H2O) and add to the medium after autoclaving. Calcium carbonate in the medium serves as a buffer. The calcium carbonate will settle in agar plates before the agar has set, producing an opaque layer in the bottom. As the strain grows and acid is produced this will react with the calcium carbonate, causing it to dissolve and form zones of clearing immediately below the colonies.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:09.961896Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 3
2026-03-13T06:19:04.823729Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001510
2026-03-17T06:29:47.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:16.891271+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "AZOTOBACTER MEDIUM" to "azotobacter_medium" for consistent matching
2026-06-05T07:40:50.171762+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:53.679316+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T10:55:41.454416+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.