Overview
- External ontology
- mediadive.medium:405 (source link unavailable) — SP2 MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium405.pdf
Composition
flowchart LR
CultureMech_001515["sp2_medium"]
ing0_Casitone["Casitone"]
CultureMech_001515 --> ing0_Casitone
ing1_Yeast_extract["Yeast extract"]
CultureMech_001515 --> ing1_Yeast_extract
ing2_Na_acetate["Na-acetate"]
CultureMech_001515 --> ing2_Na_acetate
CHEBI_32954["CHEBI:32954"]
ing2_Na_acetate -.-> CHEBI_32954
ing3_Agar["Agar"]
CultureMech_001515 --> ing3_Agar
CHEBI_2509["CHEBI:2509"]
ing3_Agar -.-> CHEBI_2509
ing4_NaCl["NaCl"]
CultureMech_001515 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_KCl["KCl"]
CultureMech_001515 --> ing5_KCl
CHEBI_32588["CHEBI:32588"]
ing5_KCl -.-> CHEBI_32588
ing6_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001515 --> ing6_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing6_MgSO4_x_7_H2O -.-> CHEBI_31795
ing7_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001515 --> ing7_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing7_MgCl2_x_6_H2O -.-> CHEBI_86345
ing8_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001515 --> ing8_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing8_CaCl2_x_2_H2O -.-> CHEBI_86158
ing9_NaHCO3["NaHCO3"]
CultureMech_001515 --> ing9_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing9_NaHCO3 -.-> CHEBI_32139
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Casitone | 1 G_PER_L | ||
| 2 | Yeast extract | 0.2 G_PER_L | ||
| 3 |
Na-acetate
C2H3O2.Na 82.034 g/mol
|
0.02 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 4 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
(if required) |
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
24.7 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 6 |
KCl
Cl.K 74.551 g/mol
|
0.7 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 7 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
6.3 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 8 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
4.6 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 9 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1.3 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 10 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.2 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Preparation
- Adjust pH to 7.2.
- If natural sea water is used, the pH need not be adjusted.
- To prevent precipitation, CaCl2 and NaHCO3 are autoclaved each as separate stock solutions in 10 ml distilled water.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.063232Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 405 |
| 2026-03-13T06:19:04.864979Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001515 |
| 2026-03-17T06:30:33.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:16.935728+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SP2 MEDIUM" to "sp2_medium" for consistent matching |
| 2026-06-05T07:41:46.113115+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:11.570887+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |