Overview
- External ontology
- mediadive.medium:411 (source link unavailable) — CLOSTRIDIUM ACETOBUTYLICUM MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.8
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium411.pdf
Composition
flowchart LR
CultureMech_001520["clostridium_acetobutylicum_medium"]
ing0_Potato["Potato"]
CultureMech_001520 --> ing0_Potato
ing1_CaCO3["CaCO3"]
CultureMech_001520 --> ing1_CaCO3
CHEBI_3311["CHEBI:3311"]
ing1_CaCO3 -.-> CHEBI_3311
ing2_Sodium_resazurin["Sodium resazurin"]
CultureMech_001520 --> ing2_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing2_Sodium_resazurin -.-> CHEBI_8806
ing3_D_Glucose["D-Glucose"]
CultureMech_001520 --> ing3_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing3_D_Glucose -.-> CHEBI_17634
ing4_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_001520 --> ing4_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing4_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing5_Milk["Milk"]
CultureMech_001520 --> ing5_Milk
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Potato | 200 G_PER_L | ||
| 2 |
CaCO3
CO3.Ca 100.086 g/mol
|
2 G_PER_L |
CHEBI:3311
calcium carbonate
|
|
| 3 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.0006000000000000001 G_PER_L |
CHEBI:8806
Resazurin
|
[Merged 2 duplicates: 0.0005, 0.0001] |
| 4 |
D-Glucose
C6H12O6 180.156 g/mol
|
6 G_PER_L |
CHEBI:17634
D-glucose
|
|
| 5 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 6 | Milk | 100 G_PER_L |
Preparation
- Mix potatoes and CaCO3 then autoclave 30 min at 121°C. Filter through cheese cloth, add glucose and cysteine, distribute under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave at 121°C for 20 min. Adjust pH of complete medium to 6.8.
- Note: For the reactivation of freeze-dried strains it is recommended to use the following simple milk medium:
- Adjust pH to 7.1. Tube and autoclave under 100% N2 gas atmosphere for 12 min at 121°C.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.074513Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 411 |
| 2026-03-13T03:08:59.347042Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:04.921008Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001520 |
| 2026-03-17T06:30:04.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:16.994685+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CLOSTRIDIUM ACETOBUTYLICUM MEDIUM" to "clostridium_acetobutylicum_medium" for consistent matching |
| 2026-06-05T07:40:53.691961+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |