Overview
- External ontology
- mediadive.medium:414 (source link unavailable) — ACIDAMINOCOCCUS FERMENTANS MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 7.0, 'max': 7.2}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium414.pdf
Composition
flowchart LR
CultureMech_001523["acidaminococcus_fermentans_medium"]
ing0_Trypticase_peptone["Trypticase peptone"]
CultureMech_001523 --> ing0_Trypticase_peptone
ing1_Casamino_acids["Casamino acids"]
CultureMech_001523 --> ing1_Casamino_acids
ing2_Yeast_extract["Yeast extract"]
CultureMech_001523 --> ing2_Yeast_extract
ing3_Glucose["Glucose"]
CultureMech_001523 --> ing3_Glucose
CHEBI_17234["CHEBI:17234"]
ing3_Glucose -.-> CHEBI_17234
ing4_Na_glutamate["Na glutamate"]
CultureMech_001523 --> ing4_Na_glutamate
CHEBI_64220["CHEBI:64220"]
ing4_Na_glutamate -.-> CHEBI_64220
ing5_Arginine["Arginine"]
CultureMech_001523 --> ing5_Arginine
CHEBI_29016["CHEBI:29016"]
ing5_Arginine -.-> CHEBI_29016
ing6_Glycine["Glycine"]
CultureMech_001523 --> ing6_Glycine
CHEBI_15428["CHEBI:15428"]
ing6_Glycine -.-> CHEBI_15428
ing7_DL_Tryptophan["DL-Tryptophan"]
CultureMech_001523 --> ing7_DL_Tryptophan
CHEBI_27897["CHEBI:27897"]
ing7_DL_Tryptophan -.-> CHEBI_27897
ing8_KH2PO4["KH2PO4"]
CultureMech_001523 --> ing8_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing8_KH2PO4 -.-> CHEBI_63036
ing9_Tween_80["Tween 80"]
CultureMech_001523 --> ing9_Tween_80
CHEBI_53426["CHEBI:53426"]
ing9_Tween_80 -.-> CHEBI_53426
ing10_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_001523 --> ing10_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing10_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Trypticase peptone | 5 G_PER_L | ||
| 2 | Casamino acids | 10 G_PER_L | ||
| 3 | Yeast extract | 5 G_PER_L | ||
| 4 |
Glucose
C6H12O6 180.156 g/mol
|
5 G_PER_L |
CHEBI:17234
glucose
|
|
| 5 |
Na glutamate
C5H8NO4.Na 169.112 g/mol
|
4 G_PER_L |
CHEBI:64220
monosodium glutamate
|
|
| 6 |
Arginine
C6H14N4O2 174.204 g/mol
|
1 G_PER_L |
CHEBI:29016
arginine
|
|
| 7 |
Glycine
C2H5NO2 75.067 g/mol
|
1 G_PER_L |
CHEBI:15428
glycine
|
|
| 8 |
DL-Tryptophan
C11H12N2O2 204.229 g/mol
|
0.1 G_PER_L |
CHEBI:27897
tryptophan
|
|
| 9 |
KH2PO4
H2O4P.K 136.084 g/mol
|
2 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 10 |
Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
|
0.5 G_PER_L |
CHEBI:53426
polysorbate 80
|
|
| 11 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Preparation
- pH 7.0 - 7.2.
- Prepare and distribute medium anaerobically using the Hungate technique
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.083828Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 414 |
| 2026-01-28T19:48:31.890101Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:04.951221Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001523 |
| 2026-03-17T06:30:33.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:17.030158+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ACIDAMINOCOCCUS FERMENTANS MEDIUM" to "acidaminococcus_fermentans_medium" for consistent matching |
| 2026-05-20T06:01:07.284938+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '7-7.2' -> {'min': 7.0, 'max': 7.2} |
| 2026-06-05T07:40:47.008554+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:35.431537+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T07:46:52.483431+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-07T10:55:41.454416+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |