oxalobacter_medium

CultureMech:001524 bacterial COMPLEX LIQUID
Original name: OXALOBACTER MEDIUM

Overview

External ontology
mediadive.medium:419 (source link unavailable) — OXALOBACTER MEDIUM
Applications
  • Microbial cultivation
pH
6.8
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium419.pdf

Composition

flowchart LR
    CultureMech_001524["oxalobacter_medium"]
    ing0_K2HPO4["K2HPO4"]
    CultureMech_001524 --> ing0_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing0_K2HPO4 -.-> CHEBI_131527
    ing1_KH2PO4["KH2PO4"]
    CultureMech_001524 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2__NH4_2SO4["(NH4)2SO4"]
    CultureMech_001524 --> ing2__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing2__NH4_2SO4 -.-> CHEBI_62946
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001524 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_Na_oxalate["Na-oxalate"]
    CultureMech_001524 --> ing4_Na_oxalate
    CHEBI_132764["CHEBI:132764"]
    ing4_Na_oxalate -.-> CHEBI_132764
    ing5_Na_acetate["Na-acetate"]
    CultureMech_001524 --> ing5_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing5_Na_acetate -.-> CHEBI_32954
    ing6_Yeast_extract["Yeast extract"]
    CultureMech_001524 --> ing6_Yeast_extract
    ing7_Trypticase_peptone["Trypticase peptone"]
    CultureMech_001524 --> ing7_Trypticase_peptone
    ing8_Sodium_resazurin["Sodium resazurin"]
    CultureMech_001524 --> ing8_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing8_Sodium_resazurin -.-> CHEBI_8806
    ing9_Na2CO3["Na2CO3"]
    CultureMech_001524 --> ing9_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing9_Na2CO3 -.-> CHEBI_29377
    ing10_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_001524 --> ing10_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing10_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing11_HCl["HCl"]
    CultureMech_001524 --> ing11_HCl
    CHEBI_17883["CHEBI:17883"]
    ing11_HCl -.-> CHEBI_17883
    ing12_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_001524 --> ing12_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing12_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing13_ZnCl2["ZnCl2"]
    CultureMech_001524 --> ing13_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing13_ZnCl2 -.-> CHEBI_49976
    ing14_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001524 --> ing14_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing14_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing15_H3BO3["H3BO3"]
    CultureMech_001524 --> ing15_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing15_H3BO3 -.-> CHEBI_33118
    ing16_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001524 --> ing16_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing16_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing17_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001524 --> ing17_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing17_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing18_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001524 --> ing18_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing18_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing19_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001524 --> ing19_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing19_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing20_NaOH["NaOH"]
    CultureMech_001524 --> ing20_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing20_NaOH -.-> CHEBI_32145
    ing21_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_001524 --> ing21_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing21_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing22_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_001524 --> ing22_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing22_Na2WO4_x_2_H2O -.-> CHEBI_63939

Ingredients (23)

# Ingredient Concentration Ontology Notes
1 K2HPO4
HO4P.2K 174.174 g/mol
0.249501 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
2 KH2PO4
H2O4P.K 136.084 g/mol
0.249501 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.499002 G_PER_L CHEBI:62946
ammonium sulfate
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.0249501 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 Na-oxalate
C2O4.2Na 133.998 g/mol
4.99002 G_PER_L CHEBI:132764
sodium oxalate
6 Na-acetate
C2H3O2.Na 82.034 g/mol
0.818363 G_PER_L CHEBI:32954
sodium acetate
7 Yeast extract 0.998004 G_PER_L
8 Trypticase peptone 0.998004 G_PER_L
9 Sodium resazurin
C12H7NO4 229.191 g/mol
0.000499002 G_PER_L CHEBI:8806
Resazurin
10 Na2CO3
CO3.2Na 105.988 g/mol
3.99202 G_PER_L CHEBI:29377
sodium carbonate
11 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.499002 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
12 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
13 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
14 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
15 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
16 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
17 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
18 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
19 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
20 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate
21 NaOH
HO.Na 39.997 g/mol
0.5 G_PER_L CHEBI:32145
sodium hydroxide
22 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.003 G_PER_L CHEBI:131361
disodium selenite pentahydrate
23 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.004 G_PER_L CHEBI:63939
sodium tungstate dihydrate

Preparation

  1. Dissolve ingredients except carbonate and cysteine, adjust pH to 6.8, bring medium to the boil and then cool to room temperature under 100% CO2 gas atmosphere. Add solid carbonate and cysteine, then equilibrate pH to 6.8. Dispense under 100% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave.
  2. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:11.086006Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 419
2026-03-13T06:19:04.960163Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001524
2026-03-17T06:30:05.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:17.040009+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "OXALOBACTER MEDIUM" to "oxalobacter_medium" for consistent matching
2026-06-05T07:41:40.681153+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 20 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:09.823760+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 7 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.