Overview
- External ontology
- mediadive.medium:429 (source link unavailable) — COLUMBIA BLOOD AGAR
- Applications
-
- Microbial cultivation
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium429.pdf Commercial Product: Brain Heart Infusion Supplier: Difco/BD Catalog: 237500 Source: https://microbenotes.com/brain-heart-infusion-bhi-agar/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_001533["columbia_blood_agar"]
ing0_Columbia_agar_base["Columbia agar base"]
CultureMech_001533 --> ing0_Columbia_agar_base
ing1_Horse_blood["Horse blood"]
CultureMech_001533 --> ing1_Horse_blood
ing2_Casein_peptone["Casein peptone"]
CultureMech_001533 --> ing2_Casein_peptone
ing3_Soy_peptone["Soy peptone"]
CultureMech_001533 --> ing3_Soy_peptone
ing4_D____Glucose["D(+)-Glucose"]
CultureMech_001533 --> ing4_D____Glucose
CHEBI_17634["CHEBI:17634"]
ing4_D____Glucose -.-> CHEBI_17634
ing5_NaCl["NaCl"]
CultureMech_001533 --> ing5_NaCl
CHEBI_26710["CHEBI:26710"]
ing5_NaCl -.-> CHEBI_26710
ing6_K2HPO4["K2HPO4"]
CultureMech_001533 --> ing6_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing6_K2HPO4 -.-> CHEBI_131527
ing7_Calf_brains["Calf brains"]
CultureMech_001533 --> ing7_Calf_brains
ing8_Beef_heart["Beef heart"]
CultureMech_001533 --> ing8_Beef_heart
ing9_Proteose_peptone["Proteose peptone"]
CultureMech_001533 --> ing9_Proteose_peptone
ing10_Dextrose["Dextrose"]
CultureMech_001533 --> ing10_Dextrose
ing10_Dextrose -.-> CHEBI_17634
ing11_Sodium_chloride["Sodium chloride"]
CultureMech_001533 --> ing11_Sodium_chloride
ing11_Sodium_chloride -.-> CHEBI_26710
ing12_Disodium_phosphate["Disodium phosphate"]
CultureMech_001533 --> ing12_Disodium_phosphate
CHEBI_34683["CHEBI:34683"]
ing12_Disodium_phosphate -.-> CHEBI_34683
ing13_Peptone["Peptone"]
CultureMech_001533 --> ing13_Peptone
ing14_Meat_extract["Meat extract"]
CultureMech_001533 --> ing14_Meat_extract
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Columbia agar base | 1000 G_PER_L | ||
| 2 | Horse blood | 40 G_PER_L | ||
| 3 | Casein peptone | 17 G_PER_L | ||
| 4 | Soy peptone | 3 G_PER_L | ||
| 5 |
D(+)-Glucose
C6H12O6 180.156 g/mol
|
2.5 G_PER_L |
CHEBI:17634
D-glucose
|
|
| 6 |
NaCl
Cl.Na 58.443 g/mol
|
5 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 7 |
K2HPO4
HO4P.2K 174.174 g/mol
|
2.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 8 |
Calf brains
Difco/BD Brain Heart Infusion 237500
|
variable VARIABLE |
UBERON:0000955
brain
|
infusion from 200 g/L; Tissue infusion - source material is calf brain tissue; Constituent of Brain Heart Infusion commercial product |
| 9 |
Beef heart
Difco/BD Brain Heart Infusion 237500
|
variable VARIABLE |
UBERON:0000948
heart
|
infusion from 250 g/L; Tissue infusion - source material is beef/ox heart tissue; Constituent of Brain Heart Infusion commercial product |
| 10 |
Proteose peptone
Difco/BD Brain Heart Infusion 237500
|
10.0 G_PER_L |
MICRO:0000180 (source link unavailable)
proteose peptone
|
Enzymatic digest of animal protein providing amino acids and peptides; Constituent of Brain Heart Infusion commercial product |
| 11 |
Dextrose
C6H12O6 180.156 g/mol
Difco/BD Brain Heart Infusion 237500
|
2.0 G_PER_L |
CHEBI:17634
D-glucose
|
Carbon/energy source; Constituent of Brain Heart Infusion commercial product |
| 12 |
Sodium chloride
Cl.Na 58.443 g/mol
Difco/BD Brain Heart Infusion 237500
|
5.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium; Constituent of Brain Heart Infusion commercial product |
| 13 |
Disodium phosphate
HO4P.2Na 141.958 g/mol
Difco/BD Brain Heart Infusion 237500
|
2.5 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
pH buffer (final pH 7.4 ± 0.2); Constituent of Brain Heart Infusion commercial product |
| 14 | Peptone | 5 G_PER_L | ||
| 15 | Meat extract | 3 G_PER_L |
Preparation
- Columbia agar base supplemented with 4% horse blood. Rehydrate pellets of freeze-dried ampoules in liquid medium 545, 215 or 1 (see below). For rehydration of Helicobacter and Campylobacter spp., use medium 215 (brain heart infusion).
- Adjust pH to 7.3.
- The same as Caso Bouillon (Merck or Oxoid, Germany).
- Adjust pH to 7.0.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.105039Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 429 |
| 2026-03-13T06:19:05.012363Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001533 |
| 2026-03-17T06:30:49.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:17.096481+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "COLUMBIA BLOOD AGAR" to "columbia_blood_agar" for consistent matching |
| 2026-06-05T07:40:54.644278+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:54.943688+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-06T00:00:00-07:00 | repair_columbia_blood_score40.py | LINKED_COLUMBIA_BLOOD_SCORE40_SOURCE_DUPLICATE | Added or refreshed reciprocal source-duplicate link for bacterial/TOGO_M2362_Columbia_Blood_Agar.yaml. |