Overview
- External ontology
- mediadive.medium:429d (source link unavailable) — FRANCISELLA HALIOTICIDA MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium429d.pdf Commercial Product: Brain Heart Infusion Supplier: Difco/BD Catalog: 237500 Source: https://microbenotes.com/brain-heart-infusion-bhi-agar/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_001537["francisella_halioticida_medium"]
ing0_Columbia_agar_base["Columbia agar base"]
CultureMech_001537 --> ing0_Columbia_agar_base
ing1_Agar["Agar"]
CultureMech_001537 --> ing1_Agar
CHEBI_2509["CHEBI:2509"]
ing1_Agar -.-> CHEBI_2509
ing2_Horse_blood["Horse blood"]
CultureMech_001537 --> ing2_Horse_blood
ing3_Glucose["Glucose"]
CultureMech_001537 --> ing3_Glucose
CHEBI_17234["CHEBI:17234"]
ing3_Glucose -.-> CHEBI_17234
ing4_FeCl3["FeCl3"]
CultureMech_001537 --> ing4_FeCl3
CHEBI_30808["CHEBI:30808"]
ing4_FeCl3 -.-> CHEBI_30808
ing5_Cysteine["Cysteine"]
CultureMech_001537 --> ing5_Cysteine
CHEBI_15356["CHEBI:15356"]
ing5_Cysteine -.-> CHEBI_15356
ing6_Casein_peptone["Casein peptone"]
CultureMech_001537 --> ing6_Casein_peptone
ing7_Soy_peptone["Soy peptone"]
CultureMech_001537 --> ing7_Soy_peptone
ing8_NaCl["NaCl"]
CultureMech_001537 --> ing8_NaCl
CHEBI_26710["CHEBI:26710"]
ing8_NaCl -.-> CHEBI_26710
ing9_Calf_brains["Calf brains"]
CultureMech_001537 --> ing9_Calf_brains
ing10_Beef_heart["Beef heart"]
CultureMech_001537 --> ing10_Beef_heart
ing11_Proteose_peptone["Proteose peptone"]
CultureMech_001537 --> ing11_Proteose_peptone
ing12_Dextrose["Dextrose"]
CultureMech_001537 --> ing12_Dextrose
CHEBI_17634["CHEBI:17634"]
ing12_Dextrose -.-> CHEBI_17634
ing13_Sodium_chloride["Sodium chloride"]
CultureMech_001537 --> ing13_Sodium_chloride
ing13_Sodium_chloride -.-> CHEBI_26710
ing14_Disodium_phosphate["Disodium phosphate"]
CultureMech_001537 --> ing14_Disodium_phosphate
CHEBI_34683["CHEBI:34683"]
ing14_Disodium_phosphate -.-> CHEBI_34683
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Columbia agar base | 700 G_PER_L | ||
| 2 | Agar | 17.0 G_PER_L |
CHEBI:2509
agar
|
[Merged 2 duplicates: 2.0, 15.0] |
| 3 | Horse blood | 40 G_PER_L | ||
| 4 |
Glucose
C6H12O6 180.156 g/mol
|
10 G_PER_L |
CHEBI:17234
glucose
|
|
| 5 |
FeCl3
Cl3Fe 162.204 g/mol
|
0.3244 G_PER_L |
CHEBI:30808
iron trichloride
|
|
| 6 |
Cysteine
C3H7NO2S 121.161 g/mol
|
1 G_PER_L |
CHEBI:15356
cysteine
|
|
| 7 | Casein peptone | 15 G_PER_L | ||
| 8 | Soy peptone | 5 G_PER_L | ||
| 9 |
NaCl
Cl.Na 58.443 g/mol
|
5 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 10 |
Calf brains
Difco/BD Brain Heart Infusion 237500
|
variable VARIABLE |
UBERON:0000955
brain
|
infusion from 200 g/L; Tissue infusion - source material is calf brain tissue; Constituent of Brain Heart Infusion commercial product |
| 11 |
Beef heart
Difco/BD Brain Heart Infusion 237500
|
variable VARIABLE |
UBERON:0000948
heart
|
infusion from 250 g/L; Tissue infusion - source material is beef/ox heart tissue; Constituent of Brain Heart Infusion commercial product |
| 12 |
Proteose peptone
Difco/BD Brain Heart Infusion 237500
|
10.0 G_PER_L |
MICRO:0000180 (source link unavailable)
proteose peptone
|
Enzymatic digest of animal protein providing amino acids and peptides; Constituent of Brain Heart Infusion commercial product |
| 13 |
Dextrose
C6H12O6 180.156 g/mol
Difco/BD Brain Heart Infusion 237500
|
2.0 G_PER_L |
CHEBI:17634
D-glucose
|
Carbon/energy source; Constituent of Brain Heart Infusion commercial product |
| 14 |
Sodium chloride
Cl.Na 58.443 g/mol
Difco/BD Brain Heart Infusion 237500
|
5.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium; Constituent of Brain Heart Infusion commercial product |
| 15 |
Disodium phosphate
HO4P.2Na 141.958 g/mol
Difco/BD Brain Heart Infusion 237500
|
2.5 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
pH buffer (final pH 7.4 ± 0.2); Constituent of Brain Heart Infusion commercial product |
Preparation
- Dissolve Columbia agar base in 70% artificial seawater and add additional 2 g agar per liter. Autoclave and mix solution with 4% horse or sheep blood after cooling to 50°C. Add 1% glucose, 2 mM FeCl3 and 0.1% cysteine, mix well and pour plates. Rehydrate pellets of freeze-dried ampoules in liquid medium 220 or 215.
- Adjust pH to 7.3. Medium is identical with Tryptone Soya Agar (Oxoid CM 131).
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.111894Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 429d |
| 2026-03-13T03:08:46.093206Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:05.033559Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001537 |
| 2026-03-17T06:29:49.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:17.117383+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "FRANCISELLA HALIOTICIDA MEDIUM" to "francisella_halioticida_medium" for consistent matching |
| 2026-06-05T07:41:05.403978+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |