kdm_2_medium

CultureMech:001543 bacterial COMPLEX SOLID_AGAR
Original name: KDM-2 MEDIUM

Overview

External ontology
mediadive.medium:435 (source link unavailable) — KDM-2 MEDIUM
Applications
  • Microbial cultivation
pH
6.5
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium435.pdf

Composition

flowchart LR
    CultureMech_001543["kdm_2_medium"]
    ing0_Peptone["Peptone"]
    CultureMech_001543 --> ing0_Peptone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_001543 --> ing1_Yeast_extract
    ing2_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_001543 --> ing2_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing2_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing3_Agar["Agar"]
    CultureMech_001543 --> ing3_Agar
    CHEBI_2509["CHEBI:2509"]
    ing3_Agar -.-> CHEBI_2509
    ing4_Activated_charcoal["Activated charcoal"]
    CultureMech_001543 --> ing4_Activated_charcoal
    ing5_Fetal_bovine_serum["Fetal bovine serum"]
    CultureMech_001543 --> ing5_Fetal_bovine_serum

Ingredients (6)

# Ingredient Concentration Ontology Notes
1 Peptone 10 G_PER_L MICRO:0000178 (source link unavailable)
Peptone
2 Yeast extract 0.5 G_PER_L FOODON:03315426
Yeast extract
3 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
1 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
4 Agar 15 G_PER_L CHEBI:2509
agar
5 Activated charcoal 2 G_PER_L
6 Fetal bovine serum 200 G_PER_L

Preparation

  1. Adjust pH to 6.5.
  2. Dissolve peptone, yeast extract and cysteine in 800 ml of distilled water. Adjust pH to 6.5 with NaOH. Add agar and dissolve by heating.
  3. Add 2 g/l activated charcoal to the agar base solution before autoclaving. After autoclaving, cool to 45°C and add sterilized serum.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:11.128331Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 435
2026-03-13T06:19:05.083500Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001543
2026-03-17T06:30:40.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:17.171658+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "KDM-2 MEDIUM" to "kdm_2_medium" for consistent matching
2026-06-05T07:41:10.358961+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-13T00:00:00-07:00 repair_bacterial_score10_exact_terms_batch3.py GROUNDED_BACTERIAL_SCORE10_EXACT_TERMS_BATCH3 Applied local mappings for Peptone, Yeast extract.
2026-10-07T10:55:41.454416+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.