ruminococcus_albus_medium

Overview

External ontology

mediadive.medium:436 (source link unavailable) — RUMINOCOCCUS ALBUS MEDIUM

Applications

• Microbial cultivation

pH

7.0

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium436.pdf Superseded historical provenance, not a current formulation claim: Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification. Preserve original preparation descriptions, but withdraw the incorrect HEAT encoding of incubation: Boil and cool under CO2. Under a gentle stream of CO2 add: Adjust pH to 7.0, distribute under N2 into appropriate vessels and sterilize. Inoculate under CO2 and incubate at 37°C. The original supplies 920 ml water, two 40 ml mineral inputs and a separate 1 ml acid input. Their sum is not an explicit final volume. Literal mineral percentages have no declared basis; no g/L or molarity is inferred. Complete source rows determine the reconstructed formulation. Archive the prior flattened nodes, unsupported LB additions and stock links in append-only history. Unrelated annotations are preserved, not recertified.

Composition

flowchart LR
    CultureMech_001544["ruminococcus_albus_medium"]
    ing0_Tryptone["Tryptone"]
    CultureMech_001544 --> ing0_Tryptone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_001544 --> ing1_Yeast_extract
    ing2_Glucose["Glucose"]
    CultureMech_001544 --> ing2_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing2_Glucose -.-> CHEBI_17234
    ing3_Cellobiose["Cellobiose"]
    CultureMech_001544 --> ing3_Cellobiose
    CHEBI_17057["CHEBI:17057"]
    ing3_Cellobiose -.-> CHEBI_17057
    ing4_Resazurin["Resazurin"]
    CultureMech_001544 --> ing4_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing4_Resazurin -.-> CHEBI_8806
    ing5_Distilled_water["Distilled water"]
    CultureMech_001544 --> ing5_Distilled_water
    ing6_Na2CO3["Na2CO3"]
    CultureMech_001544 --> ing6_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing6_Na2CO3 -.-> CHEBI_29377
    ing7_Cysteine_HCl_x_H2O["Cysteine-HCl x H2O"]
    CultureMech_001544 --> ing7_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing7_Cysteine_HCl_x_H2O -.-> CHEBI_91248

Ingredients (8)

Solutions

Target organisms

No target organisms are recorded.

Provenance