Overview
- External ontology
- mediadive.medium:437 (source link unavailable) — MITSUOKELLA DENTALIS MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium437.pdf
Composition
flowchart LR
CultureMech_001545["mitsuokella_dentalis_medium"]
ing0_Beef_extract["Beef extract"]
CultureMech_001545 --> ing0_Beef_extract
ing1_Trypticase["Trypticase"]
CultureMech_001545 --> ing1_Trypticase
ing2_Yeast_extract["Yeast extract"]
CultureMech_001545 --> ing2_Yeast_extract
ing3__NH4_2SO4["(NH4)2SO4"]
CultureMech_001545 --> ing3__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing3__NH4_2SO4 -.-> CHEBI_62946
ing4_Glucose["Glucose"]
CultureMech_001545 --> ing4_Glucose
CHEBI_17234["CHEBI:17234"]
ing4_Glucose -.-> CHEBI_17234
ing5_Resazurin["Resazurin"]
CultureMech_001545 --> ing5_Resazurin
CHEBI_8806["CHEBI:8806"]
ing5_Resazurin -.-> CHEBI_8806
ing6_Haemin["Haemin"]
CultureMech_001545 --> ing6_Haemin
CHEBI_50385["CHEBI:50385"]
ing6_Haemin -.-> CHEBI_50385
ing7_Vitamin_K1["Vitamin K1"]
CultureMech_001545 --> ing7_Vitamin_K1
CHEBI_18067["CHEBI:18067"]
ing7_Vitamin_K1 -.-> CHEBI_18067
ing8_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_001545 --> ing8_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing8_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing9_Bovine_serum["Bovine serum"]
CultureMech_001545 --> ing9_Bovine_serum
ing10_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001545 --> ing10_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing10_CaCl2_x_2_H2O -.-> CHEBI_86158
ing11_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001545 --> ing11_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing11_MgSO4_x_7_H2O -.-> CHEBI_31795
ing12_K2HPO4["K2HPO4"]
CultureMech_001545 --> ing12_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing12_K2HPO4 -.-> CHEBI_131527
ing13_KH2PO4["KH2PO4"]
CultureMech_001545 --> ing13_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing13_KH2PO4 -.-> CHEBI_63036
ing14_NaHCO3["NaHCO3"]
CultureMech_001545 --> ing14_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing14_NaHCO3 -.-> CHEBI_32139
ing15_NaCl["NaCl"]
CultureMech_001545 --> ing15_NaCl
CHEBI_26710["CHEBI:26710"]
ing15_NaCl -.-> CHEBI_26710
Ingredients (16)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Beef extract | 5 G_PER_L | ||
| 2 | Trypticase | 5 G_PER_L |
MICRO:0000175 (source link unavailable)
Trypticase peptone
|
|
| 3 | Yeast extract | 10 G_PER_L | ||
| 4 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.5 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 5 |
Glucose
C6H12O6 180.156 g/mol
|
5 G_PER_L |
CHEBI:17234
glucose
|
|
| 6 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.001 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 7 |
Haemin
C34H32ClFeN4O4 651.952 g/mol
|
0.005 G_PER_L |
CHEBI:50385
hemin
|
|
| 8 |
Vitamin K1
C31H46O2 450.707 g/mol
|
0.001 G_PER_L |
CHEBI:18067
phylloquinone
|
|
| 9 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 10 | Bovine serum | 50 G_PER_L | ||
| 11 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.3 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 12 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.48 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 13 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 14 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 15 |
NaHCO3
CHO3.Na 84.006 g/mol
|
10 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 16 |
NaCl
Cl.Na 58.443 g/mol
|
2 G_PER_L |
CHEBI:26710
sodium chloride
|
Preparation
- Dissolve ingredients except cysteine-HCl in water, boil for 5 min, cool to room temperature while gassing with 100% CO2, add cysteine-HCl and adjust pH to 6.9 with 8 N NaOH while gassing is continued. Switch to 100% N2 gas after pH has reached the desired value, distribute in anaerobic tubes under nitrogen, and autoclave.
- Adjust pH to 6.9.
- Before use add 5% (v/v) bovine serum (made anaerobic by preincubation in a GasPak container over-night).
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.132155Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 437 |
| 2026-01-28T19:48:29.183686Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:05.097750Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001545 |
| 2026-03-17T06:30:27.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:17.187292+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MITSUOKELLA DENTALIS MEDIUM" to "mitsuokella_dentalis_medium" for consistent matching |
| 2026-06-05T07:41:34.824919+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:08.086834+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-22T00:03:26.686152+00:00 | repair_rejected_peptone_groundings.py | CORRECTED_REJECTED_PEPTONE_GROUNDING | CultureMech #484. /ingredients/1/term/id: Trypticase CHEBI:78018 -> MICRO:0000175 (replace_term). Reviewed identities: https://github.com/CultureBotAI/MediaIngredientMech/tree/24562493919391df4468a4285b7c997960cc8868. Publication resolver: MIM label index 9f09e4fb97fb0e6cbbd6f25baca40b36512adb88. MediaDive source IDs and concentrations preserved. |