Overview
- External ontology
- mediadive.medium:449 (source link unavailable) — M17 MEDIUM FOR LACTIC STREPTOCOCCI
- Applications
-
- Microbial cultivation
- pH range
- {'min': 6.7, 'max': 7.1}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium449.pdf
Composition
flowchart LR
CultureMech_001558["m17_medium_for_lactic_streptococci"]
ing0_Casein_peptone["Casein peptone"]
CultureMech_001558 --> ing0_Casein_peptone
ing1_Soy_peptone["Soy peptone"]
CultureMech_001558 --> ing1_Soy_peptone
ing2_Bacto_peptone["Bacto peptone"]
CultureMech_001558 --> ing2_Bacto_peptone
ing3_Yeast_extract["Yeast extract"]
CultureMech_001558 --> ing3_Yeast_extract
ing4_Ascorbic_acid["Ascorbic acid"]
CultureMech_001558 --> ing4_Ascorbic_acid
CHEBI_22652["CHEBI:22652"]
ing4_Ascorbic_acid -.-> CHEBI_22652
ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001558 --> ing5_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
ing6_Sodium_beta_glycerophosphate["Sodium beta-glycerophosphate"]
CultureMech_001558 --> ing6_Sodium_beta_glycerophosphate
CHEBI_132089["CHEBI:132089"]
ing6_Sodium_beta_glycerophosphate -.-> CHEBI_132089
ing7_Lactose["Lactose"]
CultureMech_001558 --> ing7_Lactose
CHEBI_17716["CHEBI:17716"]
ing7_Lactose -.-> CHEBI_17716
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Casein peptone | 5 G_PER_L | ||
| 2 | Soy peptone | 5 G_PER_L | ||
| 3 | Bacto peptone | 5 G_PER_L | ||
| 4 | Yeast extract | 2.5 G_PER_L | ||
| 5 | Ascorbic acid | 0.5 G_PER_L |
CHEBI:22652
ascorbic acid
|
|
| 6 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.25 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 7 |
Sodium beta-glycerophosphate
C3H7O6P.2Na 216.037 g/mol
|
19 G_PER_L |
CHEBI:132089
sodium glycerol 2-phosphate
|
|
| 8 |
Lactose
C12H22O11 342.297 g/mol
|
5 G_PER_L |
CHEBI:17716
lactose
|
Preparation
- Adjust pH to 6.9 ± 0.2.
- Lactose should be sterilized by filtration.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:11.157686Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 449 |
| 2026-03-13T06:19:05.180782Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001558 |
| 2026-03-17T06:30:15.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:17.281631+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "M17 MEDIUM FOR LACTIC STREPTOCOCCI" to "m17_medium_for_lactic_streptococci" for consistent matching |
| 2026-05-20T06:01:24.736589+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '6.7-7.1' -> {'min': 6.7, 'max': 7.1} |
| 2026-06-05T07:41:11.735282+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-10-07T10:55:41.454416+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |