eba_medium

CultureMech:001564 bacterial DEFINED LIQUID
Original name: EBA MEDIUM

Overview

External ontology
mediadive.medium:454 (source link unavailable) — EBA MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 7.2, 'max': 7.4}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium454.pdf

Composition

flowchart LR
    CultureMech_001564["eba_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_001564 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_NaCl["NaCl"]
    CultureMech_001564 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_KCl["KCl"]
    CultureMech_001564 --> ing2_KCl
    CHEBI_32588["CHEBI:32588"]
    ing2_KCl -.-> CHEBI_32588
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001564 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001564 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_NH4Cl["NH4Cl"]
    CultureMech_001564 --> ing5_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing5_NH4Cl -.-> CHEBI_31206
    ing6_Resazurin["Resazurin"]
    CultureMech_001564 --> ing6_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing6_Resazurin -.-> CHEBI_8806
    ing7_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_001564 --> ing7_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing7_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing8_Glycine["Glycine"]
    CultureMech_001564 --> ing8_Glycine
    CHEBI_15428["CHEBI:15428"]
    ing8_Glycine -.-> CHEBI_15428
    ing9_Biotin["Biotin"]
    CultureMech_001564 --> ing9_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing9_Biotin -.-> CHEBI_15956
    ing10_NaHCO3["NaHCO3"]
    CultureMech_001564 --> ing10_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing10_NaHCO3 -.-> CHEBI_32139
    ing11_Na2S["Na2S"]
    CultureMech_001564 --> ing11_Na2S
    CHEBI_76208["CHEBI:76208"]
    ing11_Na2S -.-> CHEBI_76208
    ing12_HCl["HCl"]
    CultureMech_001564 --> ing12_HCl
    CHEBI_17883["CHEBI:17883"]
    ing12_HCl -.-> CHEBI_17883
    ing13_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_001564 --> ing13_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing13_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing14_ZnCl2["ZnCl2"]
    CultureMech_001564 --> ing14_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing14_ZnCl2 -.-> CHEBI_49976
    ing15_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001564 --> ing15_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing15_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing16_H3BO3["H3BO3"]
    CultureMech_001564 --> ing16_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing16_H3BO3 -.-> CHEBI_33118
    ing17_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001564 --> ing17_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing17_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing18_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001564 --> ing18_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing18_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing19_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001564 --> ing19_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing19_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing20_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001564 --> ing20_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing20_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (21)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.209205 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 NaCl
Cl.Na 58.443 g/mol
1.04603 G_PER_L CHEBI:26710
sodium chloride
3 KCl
Cl.K 74.551 g/mol
0.523013 G_PER_L CHEBI:32588
potassium chloride
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.523013 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.156904 G_PER_L CHEBI:86158
calcium chloride dihydrate
6 NH4Cl
Cl.H4N 53.492 g/mol
0.261506 G_PER_L CHEBI:31206
ammonium chloride
7 Resazurin
C12H7NO4 229.191 g/mol
0.00104603 G_PER_L CHEBI:8806
Resazurin
8 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.00263014 G_PER_L CHEBI:131361
disodium selenite pentahydrate
9 Glycine
C2H5NO2 75.067 g/mol
2.09205 G_PER_L CHEBI:15428
glycine
10 Biotin
C10H16N2O3S 244.316 g/mol
0.2 G_PER_L CHEBI:15956
biotin
11 NaHCO3
CHO3.Na 84.006 g/mol
3 G_PER_L CHEBI:32139
sodium hydrogencarbonate
12 Na2S
2Na.S 78.047 g/mol
1 G_PER_L CHEBI:76208
sodium sulfide (anhydrous)
13 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
14 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
15 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
16 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
17 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
18 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
19 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
20 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
21 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. pH 7.2 to 7.4
  2. Before inoculation add per 100 ml of medium:
  3. Prepare and distribute medium anaerobically by using the Hungate technique.
  4. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:11.166425Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 454
2026-03-13T06:19:05.211171Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001564
2026-03-17T06:29:51.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:17.316384+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "EBA MEDIUM" to "eba_medium" for consistent matching
2026-05-20T06:01:16.019707+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '7.2-7.4' -> {'min': 7.2, 'max': 7.4}
2026-06-05T07:40:59.283447+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:56.610436+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.