kpl_medium

Overview

External ontology

mediadive.medium:456 (source link unavailable) — KPL MEDIUM

Applications

• Microbial cultivation

pH

5.5

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium456.pdf

Composition

flowchart LR
    CultureMech_001566["kpl_medium"]
    ing0_Glucose["Glucose"]
    CultureMech_001566 --> ing0_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing0_Glucose -.-> CHEBI_17234
    ing1_Galactose["Galactose"]
    CultureMech_001566 --> ing1_Galactose
    CHEBI_28260["CHEBI:28260"]
    ing1_Galactose -.-> CHEBI_28260
    ing2_Tween_80["Tween 80"]
    CultureMech_001566 --> ing2_Tween_80
    CHEBI_53426["CHEBI:53426"]
    ing2_Tween_80 -.-> CHEBI_53426
    ing3_Agar["Agar"]
    CultureMech_001566 --> ing3_Agar
    CHEBI_2509["CHEBI:2509"]
    ing3_Agar -.-> CHEBI_2509
    ing4_Skim_milk["Skim milk"]
    CultureMech_001566 --> ing4_Skim_milk
    ing5_White_wine["White wine"]
    CultureMech_001566 --> ing5_White_wine

Ingredients (6)

Preparation

1. Combine components, except white table wine. Adjust pH to 5.5. Autoclave for 15 min at 121°C, Cool to 45-50°C. Aseptically add filter sterilized white table wine. Mix thoroughly. Pour into sterile Petri dishes.
2. Adjust 10% skim milk to pH 5.5 with lactic acid and heat at 100°C for 30 minutes. Remove precipitate by filtration. For preparing broth medium, adjust filtrate to pH 7.0 with 2 N NaOH and heat at 100°C for 30 minutes. Remove precipitate by filtration. Adjust filtrate to pH 5.5 with 2 N HCl.
3. Filter-sterilize and add to autoclaved basal medium.

Target organisms

No target organisms are recorded.

Provenance