mineral_medium_with_2_chlorobenzoate

Overview

External ontology

mediadive.medium:457a (source link unavailable) — MINERAL MEDIUM WITH 2-CHLOROBENZOATE

Applications

• Microbial cultivation

pH

7.4

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium457a.pdf Commercial Product: Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Supplier: Multiple suppliers (BD, Sigma, Neogen) Catalog: Multiple (22092 Sigma, 211825 BD) Source: https://en.wikipedia.org/wiki/Tryptic_soy_broth Accessed: 2026-03-28 Constituent composition researched from product specification.

Composition

flowchart LR
    CultureMech_001568["mineral_medium_with_2_chlorobenzoate"]
    ing0_2_Chlorobenzoic_acid["2-Chlorobenzoic acid"]
    CultureMech_001568 --> ing0_2_Chlorobenzoic_acid
    ing1_Na2HPO4["Na2HPO4"]
    CultureMech_001568 --> ing1_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing1_Na2HPO4 -.-> CHEBI_34683
    ing2_KH2PO4["KH2PO4"]
    CultureMech_001568 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3__NH4_2SO4["(NH4)2SO4"]
    CultureMech_001568 --> ing3__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing3__NH4_2SO4 -.-> CHEBI_62946
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001568 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001568 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_Peptone["Peptone"]
    CultureMech_001568 --> ing6_Peptone
    ing7_Meat_extract["Meat extract"]
    CultureMech_001568 --> ing7_Meat_extract
    ing8_Agar["Agar"]
    CultureMech_001568 --> ing8_Agar
    CHEBI_2509["CHEBI:2509"]
    ing8_Agar -.-> CHEBI_2509
    ing9_Casein_peptone["Casein peptone"]
    CultureMech_001568 --> ing9_Casein_peptone
    ing10_Soy_peptone["Soy peptone"]
    CultureMech_001568 --> ing10_Soy_peptone
    ing11_NaCl["NaCl"]
    CultureMech_001568 --> ing11_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing11_NaCl -.-> CHEBI_26710
    ing12_Pancreatic_digest_of_casein["Pancreatic digest of casein"]
    CultureMech_001568 --> ing12_Pancreatic_digest_of_casein
    ing13_Peptic_digest_of_soybean_meal["Peptic digest of soybean meal"]
    CultureMech_001568 --> ing13_Peptic_digest_of_soybean_meal
    ing14_Glucose["Glucose"]
    CultureMech_001568 --> ing14_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing14_Glucose -.-> CHEBI_17234
    ing15_Sodium_chloride["Sodium chloride"]
    CultureMech_001568 --> ing15_Sodium_chloride
    ing15_Sodium_chloride -.-> CHEBI_26710
    ing16_Dipotassium_phosphate["Dipotassium phosphate"]
    CultureMech_001568 --> ing16_Dipotassium_phosphate
    CHEBI_131527["CHEBI:131527"]
    ing16_Dipotassium_phosphate -.-> CHEBI_131527
    ing17_Agar["Agar"]
    CultureMech_001568 --> ing17_Agar
    ing17_Agar -.-> CHEBI_2509
    ing18_EDTA["EDTA"]
    CultureMech_001568 --> ing18_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing18_EDTA -.-> CHEBI_4735
    ing19_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001568 --> ing19_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing19_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing20_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001568 --> ing20_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing20_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing21_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001568 --> ing21_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing21_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing22_H3BO3["H3BO3"]
    CultureMech_001568 --> ing22_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing22_H3BO3 -.-> CHEBI_33118
    ing23_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001568 --> ing23_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing23_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing24_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001568 --> ing24_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing24_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing25_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001568 --> ing25_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing25_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing26_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001568 --> ing26_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing26_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (27)

Preparation

1. Add 10.0 ml of 2-chlorobenzoic acid solution per liter autoclaved medium 457. Adjust the pH of the final medium to 7.4.
2. Dissolve 78.3 g/l 2-chlorobenzoic acid in water by slow addition of concentrated NaOH (pH should reach 7.4). Filter-sterilize the solution.
3. Adjust pH to 6.9.
4. Prepare a separate solution of the phosphates and autoclave separately. Combine the two solutions after cooling.
5. Rehydrate and cultivate lyophilized cells in complex medium (e.g. medium 1, 220 or 535). After this reactivation, cultivate in mineral medium 457 with the appropriate carbon source.
6. Adjust pH to 7.0.
7. For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation.
8. Adjust pH to 7.3. Medium is identical with Tryptone Soya Agar (Oxoid CM 131).
9. pH 7.3
10. Autoclave at 121°C for 15 min.

Target organisms

No target organisms are recorded.

Provenance