nitrogen_free_medium_for_pseudomonas_stutzeri

CultureMech:001574 bacterial COMPLEX LIQUID
Original name: NITROGEN-FREE MEDIUM FOR PSEUDOMONAS STUTZERI

Overview

External ontology
mediadive.medium:460 (source link unavailable) — NITROGEN-FREE MEDIUM FOR PSEUDOMONAS STUTZERI
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium460.pdf

Composition

flowchart LR
    CultureMech_001574["nitrogen_free_medium_for_pseudomonas_stutzeri"]
    ing0_K2HPO4["K2HPO4"]
    CultureMech_001574 --> ing0_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing0_K2HPO4 -.-> CHEBI_131527
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001574 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_NaCl["NaCl"]
    CultureMech_001574 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_Yeast_extract["Yeast extract"]
    CultureMech_001574 --> ing3_Yeast_extract
    ing4_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_001574 --> ing4_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing4_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing5_DL_Na_malate["DL-Na-malate"]
    CultureMech_001574 --> ing5_DL_Na_malate
    CHEBI_91260["CHEBI:91260"]
    ing5_DL_Na_malate -.-> CHEBI_91260

Ingredients (6)

# Ingredient Concentration Ontology Notes
1 K2HPO4
HO4P.2K 174.174 g/mol
0.5 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
2 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.2 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
3 NaCl
Cl.Na 58.443 g/mol
0.1 G_PER_L CHEBI:26710
sodium chloride
4 Yeast extract 0.2 G_PER_L
5 FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
0.015 G_PER_L CHEBI:86254
iron trichloride hexahydrate
6 DL-Na-malate 6.6 G_PER_L CHEBI:91260
disodium malate

Preparation

  1. Adjust pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:11.197973Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 460
2026-03-13T06:19:05.293649Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001574
2026-03-17T06:30:49.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:17.406666+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "NITROGEN-FREE MEDIUM FOR PSEUDOMONAS STUTZERI" to "nitrogen_free_medium_for_pseudomonas_stutzeri" for consistent matching
2026-06-05T07:41:39.777684+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:09.587022+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T10:55:41.454416+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.