mineral_medium_with_pyrrolic_acid

CultureMech:001577 bacterial DEFINED LIQUID
Original name: MINERAL MEDIUM WITH PYRROLIC ACID

Overview

External ontology
mediadive.medium:461b (source link unavailable) — MINERAL MEDIUM WITH PYRROLIC ACID
Applications
  • Microbial cultivation
High-metal flag
True
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium461b.pdf

Composition

flowchart LR
    CultureMech_001577["mineral_medium_with_pyrrolic_acid"]
    ing0_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
    CultureMech_001577 --> ing0_Na2HPO4_x_2_H2O
    CHEBI_91258["CHEBI:91258"]
    ing0_Na2HPO4_x_2_H2O -.-> CHEBI_91258
    ing1_KH2PO4["KH2PO4"]
    CultureMech_001577 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_CaCl2["CaCl2"]
    CultureMech_001577 --> ing2_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing2_CaCl2 -.-> CHEBI_3312
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001577 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_MnSO4["MnSO4"]
    CultureMech_001577 --> ing4_MnSO4
    CHEBI_86360["CHEBI:86360"]
    ing4_MnSO4 -.-> CHEBI_86360
    ing5_NH4Cl["NH4Cl"]
    CultureMech_001577 --> ing5_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing5_NH4Cl -.-> CHEBI_31206
    ing6_NaCl["NaCl"]
    CultureMech_001577 --> ing6_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing6_NaCl -.-> CHEBI_26710
    ing7_Pyrrole_2_carboxylic_acid["Pyrrole-2-carboxylic acid"]
    CultureMech_001577 --> ing7_Pyrrole_2_carboxylic_acid
    CHEBI_36751["CHEBI:36751"]
    ing7_Pyrrole_2_carboxylic_acid -.-> CHEBI_36751
    ing8_Potassium_phosphate_buffer["Potassium phosphate buffer"]
    CultureMech_001577 --> ing8_Potassium_phosphate_buffer

Ingredients (9)

# Ingredient Concentration Ontology Notes
1 Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
1.44135 G_PER_L CHEBI:91258
disodium hydrogenphosphate dihydrate
2 KH2PO4
H2O4P.K 136.084 g/mol
0.248509 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 CaCl2
Ca.2Cl 110.984 g/mol
0.00994036 G_PER_L CHEBI:3312
calcium dichloride
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.497018 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 MnSO4
Mn.O4S 151.001 g/mol
0.00994036 G_PER_L CHEBI:86360
manganese(II) sulfate
6 NH4Cl
Cl.H4N 53.492 g/mol
0.298211 G_PER_L CHEBI:31206
ammonium chloride
7 NaCl
Cl.Na 58.443 g/mol
0.0497018 G_PER_L CHEBI:26710
sodium chloride
8 Pyrrole-2-carboxylic acid
C5H5NO2 111.1 g/mol
1111 G_PER_L CHEBI:36751
pyrrole-2-carboxylic acid
9 Potassium phosphate buffer 1000 G_PER_L

Preparation

  1. Prepare and autoclave medium 461 (vitamins not necessary). Add 10 ml of pyrrolic acid solution per liter mineral medium.
  2. Dissolve and autoclave the phosphates separately. Add the vitamin solution and trace element solution after autoclaving. After mixing, pH should be 7.5.
  3. Rehydrate and cultivate the lyophilized cells in complex medium recommended for the specific strain (e.g. medium 1, 220 or 535) first. After this reactivation, cultivate on mineral medium 461 with the appropriate carbon source.
  4. Dissolve 10 mol pyrrole-2-carboxylic acid per liter potassium phosphate buffer (50 mM; pH 7.2) and sterilize the solution by membrane filtration.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:11.205669Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 461b
2026-03-13T06:19:05.316922Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001577
2026-03-17T06:30:29.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:17.432939+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MINERAL MEDIUM WITH PYRROLIC ACID" to "mineral_medium_with_pyrrolic_acid" for consistent matching
2026-06-05T07:41:34.469092+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:08.006251+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-20T19:02:54.428563+00:00 apply_mim_groundings.py Adopted MIM published grounding Grounded 1 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308).