mineral_medium_brunner_with_vitamins

CultureMech:001579 bacterial COMPLEX SOLID_AGAR
Original name: MINERAL MEDIUM (BRUNNER) WITH VITAMINS

Overview

External ontology
mediadive.medium:462 (source link unavailable) — MINERAL MEDIUM (BRUNNER) WITH VITAMINS
Applications
  • Microbial cultivation
pH
6.9
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium462.pdf Commercial Product: Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Supplier: Multiple suppliers (BD, Sigma, Neogen) Catalog: Multiple (22092 Sigma, 211825 BD) Source: https://en.wikipedia.org/wiki/Tryptic_soy_broth Accessed: 2026-03-28 Constituent composition researched from product specification.

Composition

flowchart LR
    CultureMech_001579["mineral_medium_brunner_with_vitamins"]
    ing0_Na2HPO4["Na2HPO4"]
    CultureMech_001579 --> ing0_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing0_Na2HPO4 -.-> CHEBI_34683
    ing1_KH2PO4["KH2PO4"]
    CultureMech_001579 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2__NH4_2SO4["(NH4)2SO4"]
    CultureMech_001579 --> ing2__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing2__NH4_2SO4 -.-> CHEBI_62946
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001579 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001579 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_EDTA["EDTA"]
    CultureMech_001579 --> ing5_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing5_EDTA -.-> CHEBI_4735
    ing6_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001579 --> ing6_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing6_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing7_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001579 --> ing7_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing7_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing8_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001579 --> ing8_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing8_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing9_H3BO3["H3BO3"]
    CultureMech_001579 --> ing9_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing9_H3BO3 -.-> CHEBI_33118
    ing10_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001579 --> ing10_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing10_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing11_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001579 --> ing11_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing11_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing12_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001579 --> ing12_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing12_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing13_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001579 --> ing13_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing13_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing14_p_Aminobenzoate["p-Aminobenzoate"]
    CultureMech_001579 --> ing14_p_Aminobenzoate
    CHEBI_17836["CHEBI:17836"]
    ing14_p_Aminobenzoate -.-> CHEBI_17836
    ing15_Biotin["Biotin"]
    CultureMech_001579 --> ing15_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing15_Biotin -.-> CHEBI_15956
    ing16_Nicotinic_acid["Nicotinic acid"]
    CultureMech_001579 --> ing16_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing16_Nicotinic_acid -.-> CHEBI_15940
    ing17_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_001579 --> ing17_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing17_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing18_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_001579 --> ing18_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing18_Calcium_pantothenate -.-> CHEBI_31345
    ing19_Pyridoxamine["Pyridoxamine"]
    CultureMech_001579 --> ing19_Pyridoxamine
    CHEBI_16410["CHEBI:16410"]
    ing19_Pyridoxamine -.-> CHEBI_16410
    ing20_Vitamin_B12["Vitamin B12"]
    CultureMech_001579 --> ing20_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing20_Vitamin_B12 -.-> CHEBI_176843
    ing21_Peptone["Peptone"]
    CultureMech_001579 --> ing21_Peptone
    ing22_Meat_extract["Meat extract"]
    CultureMech_001579 --> ing22_Meat_extract
    ing23_Agar["Agar"]
    CultureMech_001579 --> ing23_Agar
    CHEBI_2509["CHEBI:2509"]
    ing23_Agar -.-> CHEBI_2509
    ing24_Casein_peptone["Casein peptone"]
    CultureMech_001579 --> ing24_Casein_peptone
    ing25_Soy_peptone["Soy peptone"]
    CultureMech_001579 --> ing25_Soy_peptone
    ing26_NaCl["NaCl"]
    CultureMech_001579 --> ing26_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing26_NaCl -.-> CHEBI_26710
    ing27_Pancreatic_digest_of_casein["Pancreatic digest of casein"]
    CultureMech_001579 --> ing27_Pancreatic_digest_of_casein
    ing28_Peptic_digest_of_soybean_meal["Peptic digest of soybean meal"]
    CultureMech_001579 --> ing28_Peptic_digest_of_soybean_meal
    ing29_Glucose["Glucose"]
    CultureMech_001579 --> ing29_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing29_Glucose -.-> CHEBI_17234
    CultureMech_001579 --> more["...3 more"]

Ingredients (33)

# Ingredient Concentration Ontology Notes
1 Na2HPO4
HO4P.2Na 141.958 g/mol
2.44 G_PER_L CHEBI:34683
disodium hydrogenphosphate
2 KH2PO4
H2O4P.K 136.084 g/mol
1.52 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.5 G_PER_L CHEBI:62946
ammonium sulfate
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.2 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.05 G_PER_L CHEBI:86158
calcium chloride dihydrate
6 EDTA
C10H16N2O8 292.244 g/mol
0.5 G_PER_L CHEBI:4735
ethylenediaminetetraacetic acid
7 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.2 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
8 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
9 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.03 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
10 H3BO3
H3BO3 61.833 g/mol
0.3 G_PER_L CHEBI:33118
boric acid
11 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
12 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.01 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
13 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.02 G_PER_L CHEBI:34887
nickel dichloride
14 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.03 G_PER_L CHEBI:75213
sodium molybdate dihydrate
15 p-Aminobenzoate
C7H6NO2 136.13 g/mol
0.01 G_PER_L CHEBI:17836
4-aminobenzoate
16 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
17 Nicotinic acid
C6H5NO2 123.111 g/mol
0.02 G_PER_L CHEBI:15940
nicotinic acid
18 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.01 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate
19 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
20 Pyridoxamine
C8H12N2O2 168.196 g/mol
0.05 G_PER_L CHEBI:16410
pyridoxamine
21 Vitamin B12 0.02 G_PER_L CHEBI:176843
vitamin B12
22 Peptone 5 G_PER_L
23 Meat extract 3 G_PER_L
24 Agar 50.0 G_PER_L CHEBI:2509
agar
 (for solid medium) [Merged 3 duplicates: 15.0, 15.0, 20.0]
25 Casein peptone 15 G_PER_L
26 Soy peptone 5 G_PER_L
27 NaCl
Cl.Na 58.443 g/mol
5 G_PER_L CHEBI:26710
sodium chloride
28 Pancreatic digest of casein
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
17.0 G_PER_L MICRO:0000182 (source link unavailable)
tryptone
 Pancreatic enzymatic digest of casein providing amino acids and peptides; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
29 Peptic digest of soybean meal
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
3.0 G_PER_L FOODON:03315720
vegetable protein, hydrolyzed
 Peptic digest of soybean meal providing additional peptides and carbohydrates; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
30 Glucose
C6H12O6 180.156 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
2.5 G_PER_L CHEBI:17234
glucose
 Carbon/energy source; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
31 Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
5.0 G_PER_L CHEBI:26710
sodium chloride
 Maintains osmotic equilibrium; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
32 Dipotassium phosphate
HO4P.2K 174.174 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
2.5 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 pH buffer; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
33 Agar
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
15.0 G_PER_L CHEBI:2509
agar
 Solidifying agent (for TSA only, not in TSB); Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product

Preparation

  1. Adjust pH to 6.9.
  2. Rehydrate and cultivate lyophilized cells in the complex medium recommended for the specific strain (e.g. medium 1, 220 or 535). After this reactivation, cultivate in mineral medium 462 with the appropriate carbon source.
  3. Adjust pH to 7.0.
  4. For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation.
  5. Adjust pH to 7.3. Medium is identical with Tryptone Soya Agar (Oxoid CM 131).
  6. pH 7.3
  7. Autoclave at 121°C for 15 min.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:11.209812Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 462
2026-03-13T03:09:01.536476Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:19:05.333405Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001579
2026-03-17T06:30:06.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:17.450196+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MINERAL MEDIUM (BRUNNER) WITH VITAMINS" to "mineral_medium_brunner_with_vitamins" for consistent matching
2026-06-05T07:41:34.117154+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 23 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:07.867334+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.