plates_with_fluoranthene

CultureMech:001580 bacterial COMPLEX SOLID_AGAR
Original name: PLATES WITH FLUORANTHENE

Overview

External ontology
mediadive.medium:462a (source link unavailable) — PLATES WITH FLUORANTHENE
Applications
  • Microbial cultivation
pH
6.9
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium462a.pdf Commercial Product: Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Supplier: Multiple suppliers (BD, Sigma, Neogen) Catalog: Multiple (22092 Sigma, 211825 BD) Source: https://en.wikipedia.org/wiki/Tryptic_soy_broth Accessed: 2026-03-28 Constituent composition researched from product specification.

Composition

flowchart LR
    CultureMech_001580["plates_with_fluoranthene"]
    ing0_Fluoranthene["Fluoranthene"]
    CultureMech_001580 --> ing0_Fluoranthene
    CHEBI_33083["CHEBI:33083"]
    ing0_Fluoranthene -.-> CHEBI_33083
    ing1_Na2HPO4["Na2HPO4"]
    CultureMech_001580 --> ing1_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing1_Na2HPO4 -.-> CHEBI_34683
    ing2_KH2PO4["KH2PO4"]
    CultureMech_001580 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3__NH4_2SO4["(NH4)2SO4"]
    CultureMech_001580 --> ing3__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing3__NH4_2SO4 -.-> CHEBI_62946
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001580 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001580 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_EDTA["EDTA"]
    CultureMech_001580 --> ing6_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing6_EDTA -.-> CHEBI_4735
    ing7_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001580 --> ing7_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing7_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing8_p_Aminobenzoate["p-Aminobenzoate"]
    CultureMech_001580 --> ing8_p_Aminobenzoate
    CHEBI_17836["CHEBI:17836"]
    ing8_p_Aminobenzoate -.-> CHEBI_17836
    ing9_Biotin["Biotin"]
    CultureMech_001580 --> ing9_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing9_Biotin -.-> CHEBI_15956
    ing10_Nicotinic_acid["Nicotinic acid"]
    CultureMech_001580 --> ing10_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing10_Nicotinic_acid -.-> CHEBI_15940
    ing11_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_001580 --> ing11_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing11_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing12_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_001580 --> ing12_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing12_Calcium_pantothenate -.-> CHEBI_31345
    ing13_Pyridoxamine["Pyridoxamine"]
    CultureMech_001580 --> ing13_Pyridoxamine
    CHEBI_16410["CHEBI:16410"]
    ing13_Pyridoxamine -.-> CHEBI_16410
    ing14_Vitamin_B12["Vitamin B12"]
    CultureMech_001580 --> ing14_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing14_Vitamin_B12 -.-> CHEBI_176843
    ing15_Peptone["Peptone"]
    CultureMech_001580 --> ing15_Peptone
    ing16_Meat_extract["Meat extract"]
    CultureMech_001580 --> ing16_Meat_extract
    ing17_Agar["Agar"]
    CultureMech_001580 --> ing17_Agar
    CHEBI_2509["CHEBI:2509"]
    ing17_Agar -.-> CHEBI_2509
    ing18_Casein_peptone["Casein peptone"]
    CultureMech_001580 --> ing18_Casein_peptone
    ing19_Soy_peptone["Soy peptone"]
    CultureMech_001580 --> ing19_Soy_peptone
    ing20_NaCl["NaCl"]
    CultureMech_001580 --> ing20_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing20_NaCl -.-> CHEBI_26710
    ing21_Pancreatic_digest_of_casein["Pancreatic digest of casein"]
    CultureMech_001580 --> ing21_Pancreatic_digest_of_casein
    ing22_Peptic_digest_of_soybean_meal["Peptic digest of soybean meal"]
    CultureMech_001580 --> ing22_Peptic_digest_of_soybean_meal
    ing23_Glucose["Glucose"]
    CultureMech_001580 --> ing23_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing23_Glucose -.-> CHEBI_17234
    ing24_Sodium_chloride["Sodium chloride"]
    CultureMech_001580 --> ing24_Sodium_chloride
    ing24_Sodium_chloride -.-> CHEBI_26710
    ing25_Dipotassium_phosphate["Dipotassium phosphate"]
    CultureMech_001580 --> ing25_Dipotassium_phosphate
    CHEBI_131527["CHEBI:131527"]
    ing25_Dipotassium_phosphate -.-> CHEBI_131527
    ing26_Agar["Agar"]
    CultureMech_001580 --> ing26_Agar
    ing26_Agar -.-> CHEBI_2509
    ing27_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001580 --> ing27_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing27_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing28_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001580 --> ing28_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing28_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing29_H3BO3["H3BO3"]
    CultureMech_001580 --> ing29_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing29_H3BO3 -.-> CHEBI_33118
    CultureMech_001580 --> more["...4 more"]

Ingredients (34)

# Ingredient Concentration Ontology Notes
1 Fluoranthene
C16H10 202.256 g/mol
2 G_PER_L CHEBI:33083
fluoranthene
2 Na2HPO4
HO4P.2Na 141.958 g/mol
2.44 G_PER_L CHEBI:34683
disodium hydrogenphosphate
3 KH2PO4
H2O4P.K 136.084 g/mol
1.52 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
4 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.5 G_PER_L CHEBI:62946
ammonium sulfate
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.2 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
6 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.05 G_PER_L CHEBI:86158
calcium chloride dihydrate
7 EDTA
C10H16N2O8 292.244 g/mol
0.5 G_PER_L CHEBI:4735
ethylenediaminetetraacetic acid
8 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.2 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
9 p-Aminobenzoate
C7H6NO2 136.13 g/mol
0.01 G_PER_L CHEBI:17836
4-aminobenzoate
10 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
11 Nicotinic acid
C6H5NO2 123.111 g/mol
0.02 G_PER_L CHEBI:15940
nicotinic acid
12 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.01 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate
13 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
14 Pyridoxamine
C8H12N2O2 168.196 g/mol
0.05 G_PER_L CHEBI:16410
pyridoxamine
15 Vitamin B12 0.02 G_PER_L CHEBI:176843
vitamin B12
16 Peptone 5 G_PER_L
17 Meat extract 3 G_PER_L
18 Agar 50.0 G_PER_L CHEBI:2509
agar
 (for solid medium) [Merged 3 duplicates: 15.0, 15.0, 20.0]
19 Casein peptone 15 G_PER_L
20 Soy peptone 5 G_PER_L
21 NaCl
Cl.Na 58.443 g/mol
5 G_PER_L CHEBI:26710
sodium chloride
22 Pancreatic digest of casein
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
17.0 G_PER_L MICRO:0000182 (source link unavailable)
tryptone
 Pancreatic enzymatic digest of casein providing amino acids and peptides; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
23 Peptic digest of soybean meal
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
3.0 G_PER_L FOODON:03315720
vegetable protein, hydrolyzed
 Peptic digest of soybean meal providing additional peptides and carbohydrates; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
24 Glucose
C6H12O6 180.156 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
2.5 G_PER_L CHEBI:17234
glucose
 Carbon/energy source; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
25 Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
5.0 G_PER_L CHEBI:26710
sodium chloride
 Maintains osmotic equilibrium; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
26 Dipotassium phosphate
HO4P.2K 174.174 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
2.5 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 pH buffer; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
27 Agar
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
15.0 G_PER_L CHEBI:2509
agar
 Solidifying agent (for TSA only, not in TSB); Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
28 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
29 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.03 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
30 H3BO3
H3BO3 61.833 g/mol
0.3 G_PER_L CHEBI:33118
boric acid
31 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
32 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.01 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
33 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.02 G_PER_L CHEBI:34887
nickel dichloride
34 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.03 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Pour plates of medium 462. Dissolve 2 mg/ml fluoranthene in acetone and spread 1 ml of this solution on the surface of every pre-dried plate. Allow the acetone to evaporate under sterile conditions. Inoculate as usual.
  2. Adjust pH to 6.9.
  3. Rehydrate and cultivate lyophilized cells in the complex medium recommended for the specific strain (e.g. medium 1, 220 or 535). After this reactivation, cultivate in mineral medium 462 with the appropriate carbon source.
  4. Adjust pH to 7.0.
  5. For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation.
  6. Adjust pH to 7.3. Medium is identical with Tryptone Soya Agar (Oxoid CM 131).
  7. pH 7.3
  8. Autoclave at 121°C for 15 min.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:11.213869Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 462a
2026-03-13T03:09:12.797094Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:19:05.346912Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001580
2026-03-17T06:30:20.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:17.464573+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "PLATES WITH FLUORANTHENE" to "plates_with_fluoranthene" for consistent matching
2026-06-05T07:41:42.256989+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 23 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:10.320079+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.