reactivation_with_liquid_medium_464

CultureMech:001584 bacterial COMPLEX SOLID_AGAR
Original name: REACTIVATION WITH LIQUID MEDIUM 464

Overview

External ontology
mediadive.medium:464a (source link unavailable) — REACTIVATION WITH LIQUID MEDIUM 464
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium464a.pdf

Composition

flowchart LR
    CultureMech_001584["reactivation_with_liquid_medium_464"]
    ing0_Tryptone["Tryptone"]
    CultureMech_001584 --> ing0_Tryptone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_001584 --> ing1_Yeast_extract
    ing2_Dextrose["Dextrose"]
    CultureMech_001584 --> ing2_Dextrose
    CHEBI_17634["CHEBI:17634"]
    ing2_Dextrose -.-> CHEBI_17634
    ing3_Agar["Agar"]
    CultureMech_001584 --> ing3_Agar
    CHEBI_2509["CHEBI:2509"]
    ing3_Agar -.-> CHEBI_2509

Ingredients (4)

# Ingredient Concentration Ontology Notes
1 Tryptone 5 G_PER_L
2 Yeast extract 2.5 G_PER_L
3 Dextrose
C6H12O6 180.156 g/mol
1 G_PER_L CHEBI:17634
D-glucose
4 Agar 15 G_PER_L CHEBI:2509
agar

Preparation

  1. Rehydrate and grow lyophilized cells from the ampoule in liquid medium 464. Subsequently subculturing may be carried out in liquid medium or an agar medium.
  2. Adjust pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:11.226419Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 464a
2026-03-13T06:19:05.378973Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001584
2026-03-17T06:30:22.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:17.500706+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "REACTIVATION WITH LIQUID MEDIUM 464" to "reactivation_with_liquid_medium_464" for consistent matching
2026-06-05T07:41:44.087111+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-12T00:00:00-07:00 repair_togo_m2310_reactivation_464a_score15.py LINKED_TOGO_M2310_SOURCE_DUPLICATE Linked TOGO M2310 as a source duplicate of DSMZ Medium 464a.